Surface sterilization and micropropagation of Ludisia discolor. (November 2019)

• Record Type: Journal Article
• Authors: Poobathy, Ranjetta; Zakaria, Rahmad; Murugaiyah, Vikneswaran; Subramaniam, Sreeramanan
• Is Part Of: Biocatalysis and agricultural biotechnology. Number 22(2019)
• Publication Date: 2019-11
• Subjects: Micropropagation -- Jewel orchid -- Ludisia discolor -- Surface sterilization -- Acclimatization; HgCl2 mercury chloride -- MS Murashige and Skoog -- NAA 1-naphthaleneacetic acid -- NaOCl sodium hypochlorite -- PFD photon flux density -- TDZ thidiazuron; Agricultural biotechnology -- Periodicals; Enzymes -- Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.bcab.2019.101380
• Languages: English
• ISSNs: 1878-8181

Abstract

Abstract: The jewel orchid Ludisia discolor, found in many parts of Asia, is valued due to its appearance and medicinal benefits. Existing surface sterilization protocols were found to be not successful in initiating in vitro cultures of L. discolor in this study. Hence, a surface sterilization protocol, incorporating the use of 0.4% (w/v) mercury chloride, was developed to address this issue. This method yielded the lowest contamination (7.7%) and best growth at 22.5%. Nodal segments of L. discolor produced the best growth when placed in half-strength semi-solid MS medium with 0.2% (w/v) activated charcoal, 8% (w/v) Mas banana cultivar homogenate, 3% (w/v) sucrose, 3.5 g L −1 Gelrite, 1.0 mg L −1 1-naphthaleneacetic acid, and 0.1 mg L −1 thidiazuron, a confirmation of the protocol promoted by Shiau et al. (2005). All in vitro cultures of L. discolor were successfully acclimatised under low light conditions using coconut coir, coconut husk and peat moss. Highlights: Previous sterilization methods could not establish in vitro cultures of L. discolor for this study. In this study, 0.4% (w/v) mercury chloride gave the lowest contamination (7.7%). Nodal segments of L. discolor grew best in half-strength semi-solid MS medium with 0.2% (w/v) activated charcoal, 8% (w/v) Mas banana cultivar homogenate, 3% (w/v) sucrose, 3.5 g L −1 Gelrite, 1.0 mg L −1 1-naphthaleneacetic acid, and 0.1 mg L −1 thidiazuron. All in vitro L. discolor cultures were successfully acclimatised.