Technical note: Preparation improvement of charred cadaveric viscera using sandison's rehydrating solution for histological analysis. (January 2020)

• Record Type: Journal Article
• Authors: Gentile, Guendalina; Battistini, Alessio; Andreola, Salvatore; Boracchi, Michele; Marchesi, Matteo; Tambuzzi, Stefano; Zoja, Riccardo
• Is Part Of: Forensic science international. Volume 306(2020)
• Publication Date: 2020-01
• Subjects: Charring -- Hyperthermal lesivity -- Sandison"s rehydrating solution -- Forensic pathology -- Histology; Medical jurisprudence -- Periodicals; Chemistry, Forensic -- Periodicals; Forensic Medicine -- Periodicals; Médecine légale -- Périodiques; Chimie légale -- Périodiques; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; Chemistry, Forensic; Medical jurisprudence; Electronic journals; Periodicals; Electronic journals; 614.1
• DOI: 10.1016/j.forsciint.2019.110066
• Languages: English
• ISSNs: 0379-0738

Abstract

Highlights: On the charred corpses the microscopic examination can be particularly difficult. Sandison's rehydrating solution has been applied on charred cadavers. Sandison's solution decreased artifacts in tegument, parenchymatous viscera and hollow organs in charred corpses. Abstract: In forensic evaluation of charred corpses, internal detrimental signs may result as more significant of those observed during external examination and is often arduous to state if a victim was exposed to fire before or after death. When the histological analysis of the remaining internal viscera is necessary, the massive destruction caused by the lesion, the charring and the coarctation of the samples don't allow to give further information or to determine the remaining organic components of the viscera. This limit is determined by the intrinsic characteristics of this thermal lesivity of self-maintenance even after the exitus of the subject, worsening the initial detrimental framework. The Authors, with the purpose of improving the microscopic visualization of the samples collected from cadavers with peculiar deterioration, as in case of carbonization, suggest the use of a specific technical protocol based on the use of Sandison's rehydrating solution since the samples treated with this solution showed, at microscopic examination, a substantial histological-morphological improvement.