A novel Pd2+-free highly selective and ultrasensitive fluorescent probe for detecting CO-releasing molecule-2 in live cells and zebrafish. (March 2020)

• Record Type: Journal Article
• Authors: Li, Zilu; Yu, Chen; Chen, Yanan; Zhuang, Zihan; Tian, Bin; Liu, Caiyun; Jia, Pan; Zhu, Hanchuang; Yu, Yamin; Zhang, Xue; Sheng, Wenlong; Zhu, Baocun
• Is Part Of: Dyes and pigments. Volume 174(2020)
• Publication Date: 2020-03
• Subjects: Fluorescent probe -- Carbon monoxide -- CORM-2 -- Reduction of nitro group -- Fluorescence bioimaging; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2019.108040
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: CO-releasing molecule-2 (CORM-2), as a safe donor for releasing CO, has been widely adopted to disclose the physiopathological roles of CO and used as therapeutic drug replacing gaseous CO. The accurate determination of CORM-2 levels in biological systems is central for understanding its safe use as therapeutic agent and the cellular functions of CO. In this paper, a novel fluorescent probe CPD-NB was synthesized, which provided a safe and convenient way for detecting CORM-2 without the requirement of Pd 2+ as an additional substrate. It featured a robust fluorescence response to CORM-2 with desirable sensitivity (the detection limit was as low as 4 nM), and showed high selectivity toward CORM-2 over other biologically relevant species. Moreover, with the low cytotoxicity of probe CPD-NB, the visualizations of CORM-2 changes in live cells and zebrafish were also achieved. Thus, we expected that probe CPD-NB could be as an ideal platform for safe and powerful imaging of CORM-2 in biological systems, and may thus promote the progress of CO-related research. Graphical abstract: Image 1 Highlights: The probe could detect CORM-2 without the assistance of Pd 2+ . The probe could detect CORM-2 with ultra-sensitivity (detection limit was about 4 nM). The probe displayed prominent selectivity towards CORM-2. The probe was successful achieved in imaging the fluctuations of CORM-2 in live cells and zebrafish.