Methane production from food waste via mesophilic anaerobic digestion with ethanol pre-fermentation: Methanogenic pathway and microbial community analyses. (February 2020)

• Record Type: Journal Article
• Authors: Zou, Hui; Gao, Ming; Yu, Miao; Zhang, Wenyu; Zhang, Shuang; Wu, Chuanfu; Tashiro, Yukihiro; Wang, Qunhui
• Is Part Of: Bioresource technology. Volume 297(2020)
• Publication Date: 2020-02
• Subjects: Food waste -- Stable isotope -- High-throughput sequencing -- Methanogenic pathway -- Microbial community; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122450
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: δ 13 C-Ethanol after ethanol pre-fermentation (EP) was studied. Acetotrophic pathway is still the main metabolism pathway (59.3%) after EP. EP caused the relative abundance of Clostridium increase by 7.6% than the control. Methanobacterium became the dominant species (58.9%) after EP. Abstract: To investigate the methanogenic pathway and microbial community in a mesophilic anaerobic digestion (AD) system with food waste (FW) ethanol pre-fermentation (EP), two semi-continuous AD systems were operated by feeding FW with (PSR) and without EP (control). In this study, δ 13 C-ethanol was supplemented as solo substrate for AD sludge when the reactors operation stabilized to analyze the methanogenic pathways. The results suggested that approximately 59.3% of methane was produced from acetotrophic methanogens, while 40.7% was formed by hydrogenotrophic methanogens in the PSR group. On the other hand, compared with control, methane produced via CO2 reduction pathway was increased by 4.70%. Meanwhile, the composition variations of the microbial community in AD supported the above conclusion, since the relative abundances of Clostridium and Methanobacterium were enhanced by 7.6% and 10.2%, respectively in PSR reactor. These results provided a theoretical basis for AD applications and biogas yield improvements with EP process.