Prebiotic role of softwood hemicellulose in healthy mice model. (January 2020)

• Record Type: Journal Article
• Authors: Deloule, Vivien; Boisset, Claire; Hannani, Dalil; Suau, Antonia; Le Gouellec, Audrey; Chroboczek, Jadwiga; Botté, Cyrille; Yamario-Botté, Yoshiki; Chirat, Christine; Toussaint, Bertrand
• Is Part Of: Journal of functional foods. Volume 64(2020)
• Publication Date: 2020-01
• Subjects: Softwood hemicelluloses -- Prebiotics -- Healthy mice model -- Microbiota metabolites; AIEC Adherent-invasive Escherichia coli -- AutoH UC autohydrolysate ultrafiltrated and treated with activated charcoal -- DP degree of polymerization -- FOS fructo-oligosaccharides -- GGM galactoglucomannans -- MALDI-TOF MS Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry -- PP precipitate -- RT room temperature -- SCFA short chain fatty acids -- TSWD tryptic soy broth without dextrose; Functional foods -- Analysis -- Periodicals; Food -- Biotechnology -- Periodicals; Nutrition -- Periodicals; 613.2
• DOI: 10.1016/j.jff.2019.103688
• Languages: English
• ISSNs: 1756-4646

Abstract

Graphical abstract: Highlights: Softwood hemicellulose is a rejected by-product of paper production from wood. Hemicellulose glycans stimulate the growth of B. adolescentis, but much less that of adherent-invasive E. coli . In mice fed with hemicellulose glycans, reshaping toward a less obesogenic microbiome was observed. Fermentation metabolites sustained a health of a digestive tract and a health of an immune system. These results highlight the value of hemicelluloses as a source of potential prebiotics. Abstract: We have analyzed the prebiotic effect of softwood hemicelluloses on specific bacterial species in vitro and on microbiota diversity and metabolism in healthy mice in vivo . The ethanol-precipitated glycans from the softwood hemicellulose autohydrolysate were able to stimulate in vitro the growth of Bifidobacterium adolescentis, but to a much lesser extent than that of adherent-invasive E. coli . B. adolescentis was the best producer of SCFA. When mice were fed with the ethanol precipitate fraction, the relative abundance of Bacteroidetes increased while that of Proteobacteria diminished, suggesting change towards a less obesogenic microbiome. Following treatment, lipid analysis showed a decrease in cholesterol, bile acids and free fatty acids, indicating a potential cardioprotection role. Furthermore, analysis of fermentation metabolites revealed an increase in metabolites important for immune health and well-being. Our results highlight the value of hemicelluloses as a potential source of prebiotics supporting both a healthy digestive tract and immune system.