Co-metabolic degradation of refractory dye: A metagenomic and metaproteomic study. (January 2020)

• Record Type: Journal Article
• Authors: Zhang, Qingyun; Xie, Xuehui; Liu, Yanbiao; Zheng, Xiulin; Wang, Yiqin; Cong, Junhao; Yu, Chengzhi; Liu, Na; Sand, Wolfgang; Liu, Jianshe
• Is Part Of: Environmental pollution. Volume 256(2020)
• Publication Date: 2020-01
• Subjects: Co-metabolism -- Refractory pollutants -- Metagenomics -- Metaproteomics -- Molecular mechanism; Pollution -- Periodicals; Pollution -- Environmental aspects -- Periodicals; Environmental Pollution -- Periodicals; Pollution -- Périodiques; Pollution -- Aspect de l'environnement -- Périodiques; Pollution -- Effets physiologiques -- Périodiques; Pollution; Pollution -- Environmental aspects; Periodicals; Electronic journals; 363.73
• DOI: 10.1016/j.envpol.2019.113456
• Languages: English
• ISSNs: 0269-7491

Abstract

Abstract: Fructose was utilized as an additional co-substrate to systematically investigate the molecular mechanism of its boosting effect for the degradation of refractory dye reactive black 5 (RB5) by a natural bacterial flora DDMZ1. A decolorizing rate of 98% was measured for sample YE + FRU(200) (with 3 g/L fructose additionally to yeast extract medium, 10% (v/v) inoculation size of flora DDMZ1, 200 mg/L RB5) after 48 h. This result was 21% and 77%, respectively, higher than those of samples with only yeast extract or only fructose. Fructose was found to significantly stimulated both intracellular and extracellular azoreductase secretion causing enhanced activity. Metagenomic sequencing technology was used to analyze the functional potential of genes. A label-free quantitative proteomic approach further confirmed the encoding of functional proteins by the candidate genes. Subsequently, the molecular mechanism of RB5 degradation by candidate genes and functional proteins of the dominant species were proposed. This study provides important perspectives to the molecular mechanism of co-metabolic degradation of refractory pollutants by a natural bacterial flora. Graphical abstract: Image 1 Highlights: Fructose as an additional co-substrate boosted the dye decolorization efficiency. The metagenomic results were mostly consistent with metaproteomic ones. Functional genes and proteins exhibited a strong correlation of dominant genera. Possible molecular metabolic mechanism of RB5 was proposed.