TMT-based quantitative proteomic analysis of Eriocheir sinensis hemocytes and thoracic ganglion during Spiroplasma eriocheiris infection. Issue 96 (January 2020)

• Record Type: Journal Article
• Authors: Hou, Libo; Zhou, Haifeng; Wan, Hui; Liu, Zhanghuai; Wang, Li; Cheng, Yongxu; Wu, Xugan; Gu, Wei; Wang, Wen; Meng, Qingguo
• Is Part Of: Fish & shellfish immunology. Issue 96(2020)
• Page Start: 126
• Page End: 137
• Publication Date: 2020-01
• Subjects: Piroplasma eriocheiris -- Eriocheir sinensis -- Hemocytes -- Thoracic ganglion; Fishes -- Immunology -- Periodicals; Shellfish -- Immunology -- Periodicals; Poissons -- Immunologie -- Périodiques; Crustacés -- Immunologie -- Périodiques; 571.9617
• DOI: 10.1016/j.fsi.2019.11.009
• Languages: English
• ISSNs: 1050-4648

Abstract

Abstract: Spiroplasma eriocheiris, a novel pathogen of Chinese mitten crab Eriocheir sinensis tremor disease, has led into catastrophic economic losses in aquaculture. S. eriocheiris invaded the hemocytes in the early stage, then invaded nerve tissue and caused typically paroxysmal tremors of pereiopod in the late stage of infection. The purpose of this study was to detect the infection mechanism of hemocytes in the early stage and thoracic ganglion in the late stage of S. eriocheiris infection at the protein level. Hemocytes and thoracic ganglion were collected at 24 h and 10 d after injection (the crabs with typical paroxysmal tremors of the pereiopod), respectively. TMT was performed with isobaric markers, followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). In hemocytes, 127 proteins were up-regulated and 85 proteins were down-regulated in 2747 quantified proteins. Many proteins and process including proPO system proteins, hemolymph coagulation system proteins and lectins were differently expressed in hemocytes and involved in the early immune process of E. sinensis against S. eriocheiris infection. Meanwhile, 545 significantly different expression proteins (292 down-regulated and 253 up-regulated protein including a number of immune-associated, nervous system development and signal transmission related proteins) were identified in thoracic ganglion in the late stage of S. eriocheiris infection. The qRT-PCR analysis results shown that the selected significantly changed proteins in hemocytes and thoracic ganglion were consistent with the TMT proteomics. This paper reported for the first time to study the responses of crab hemocyte and thoracic ganglion against the S. eriocheiris infection at different stages. These findings help us understand the infection mechanism of S. eriocheiris at different stage with the different tissue. Highlights: Understand the infection mechanism of S. eriocheiris at different stage with the different tissue by proteomic analysis. 212 and 545 significantly different expression proteins were identified in hemocyte and thoracic ganglion, respectively. ProPO system, nervous system development proteins etc. were identified when the S. eriocheiris infection. Some significantly changed proteins were confirmed by qRT-PCR.