Optimization of biodiesel synthesis from nonedible oil using immobilized bio-support catalysts in jacketed packed bed bioreactor by response surface methodology. (20th January 2020)

• Record Type: Journal Article
• Authors: Kumar, Dilip; Das, Tapas; Giri, Balendu Shekher; Verma, Bhawna
• Is Part Of: Journal of cleaner production. Volume 244(2020)
• Publication Date: 2020-01-20
• Subjects: Biodiesel -- Pseudomonas cepacia -- Jacketed packed bed bioreactor -- Optimization -- Central composite design; Factory and trade waste -- Management -- Periodicals; Manufactures -- Environmental aspects -- Periodicals; Déchets industriels -- Gestion -- Périodiques; Usines -- Aspect de l'environnement -- Périodiques; 628.5
• DOI: 10.1016/j.jclepro.2019.118700
• Languages: English
• ISSNs: 0959-6526

Abstract

Abstract: The present study has been focussed on biodiesel production from nonedible oil in a jacketed packed bed bioreactor (JPBBR) using immobilized (lipase from Pseudomonas cepacia ) biocatalyst under the cleaner production. It has no harmful by products. Experimental data were evaluated by response surface methodology (RSM) integrated desirability functional approach. RSM based on a, 4 factors and 5 levels central composite design (CCD) was employed to obtain the best possible combination which was found to be under the optimized conditions of enzymatic transesterification reactions, i.e. 2-propanol to oil molar ratio_5.93:1, time_24.32 h, enzyme (lipase) loading_9.46% (w/w) and temperature_49.7 °C. The actual experimental yield was 84.58%, which compared well to the maximum predicted value of 83.80% and conversion of biodiesel yield 85.14%. Reusability test shows the bio-support catalyst could retain up to ∼70% of its initial biodiesel yield after 10 cycles. The results showed that the immobilized lipase produced high biodiesel yield after 50 days and retained 78.58%, even after 60 days. This proves the beneficial role at industrial scale. Graphical abstract: Image 1060 Highlights: The performance of JPBBR running on hybrid oil to biodiesel yield was evaluated. Immobilized P. cepacia lipase presented good reusability during 10 batch cycles. ∼84% biodiesel yield was obtained in 24 h, under optimum conditions. Immobilized lipase produced 78.58% biodiesel yield after 50 days.