An assessment of the genomics, comparative genomics and cellulose degradation potential of Mucilaginibacter polytrichastri strain RG4-7. (February 2020)

• Record Type: Journal Article
• Authors: Wang, Zi Yue; Wang, Rui Xue; Zhou, Jun Shi; Cheng, Jian Fei; Li, Yan Hong
• Is Part Of: Bioresource technology. Volume 297(2020)
• Publication Date: 2020-02
• Subjects: Mucilaginibacter -- Genomic feature -- Comparative genomic analysis -- Cellulose degradation; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122389
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Mucilaginibacter polytrichastri RG4-7 had the cellulose degradation capability. High CMCase and betaglucosidase activity could be detected in RG4-7. The cellulase related genes in RG4-7 were predicted based on its genome sequence. The GH family determines cellulose degradation in RG4-7 might be GH9, GH1 and GH3. Abstract: In this study, whole genome sequencing and comparative genomic analyses were performed for Mucilaginibacter polytrichastri RG4-7 and its carboxymethyl cellulose degradation potential was assessed. The results showed that the genome of strain RG4-7 was 5.84 Mb and contained 5019 predicted genes, in which a high proportion of strain-specific genes were related to carbohydrate metabolism. The carboxymethyl cellulose (CMC) degradation and cellulase activity tests revealed the strong cellulose degradation ability, CMCase and β-glucosidase activity in strain RG4-7. Real-time RT-PCR testing of most cellulose degradation related glycoside hydrolase (GH) families showed that GH9 (OKS85969), GH1 (OKS85832), GH3 (OKS89331 and OKS85615) were significantly up-regulated when strain RG4-7 was inoculated with CMC-Na, which suggested that GH9, GH1 and GH3 might determine its cellulose degradation ability. Certainly, further research need to be done to elucidate cellulose degradation mechanisms in strain RG4-7 in order to develop its industrial application value in lignocellulosic biomass degradation and waste management.