BRCA2 loss‐of‐function germline mutations are associated with esophageal squamous cell carcinoma risk in Chinese. Issue 4 (26th August 2019)

• Record Type: Journal Article
• Authors: Ko, Josephine Mun‐Yee; Ning, Lvwen; Zhao, Xue‐Ke; Chai, Annie Wai Yeeng; Lei, Lisa Chan; Choi, Sheyne Sta Ana; Tao, Lihua; Law, Simon; Kwong, Ava; Lee, Nikki Pui‐Yue; Chan, Kin‐Tak; Lo, Anthony; Song, Xin; Chen, Pei‐Nan; Chang, Yun‐Li; Wang, Li Dong; Lung, Maria Li
• Is Part Of: International journal of cancer. Volume 146:Issue 4(2020)
• Page Start: 1042
• Page End: 1051
• Publication Date: 2019-08-26
• Subjects: esophageal cancer susceptibility gene -- synthetic lethality -- Chinese -- BRCA2 -- loss‐of‐function mutations; Cancer -- Periodicals; Cancer -- Prevention -- Periodicals; 616.994
• DOI: 10.1002/ijc.32619
• Languages: English
• ISSNs: 0020-7136

Abstract

Abstract : Esophageal squamous cell carcinoma (ESCC) occurs with highest frequency in China with over 90% mortality, highlighting the need for early detection and improved treatment strategies. We aimed to identify ESCC cancer predisposition gene(s). Our study included 4, 517 individuals. The discovery phase using whole‐exome sequencing (WES) included 186 familial ESCC patients from high‐risk China. Targeted gene sequencing validation of 598 genes included 3, 289 Henan and 1, 228 moderate‐risk Hong Kong Chinese. A WES approach identified BRCA2 loss‐of‐function (LOF) mutations in 3.23% (6/186) familial ESCC patients compared to 0.21% (9/4300) in the ExAC East Asians (odds ratio [OR] = 15.89, p = 2.48 × 10 −10 ). BRCA2 LOF mutation frequency in the combined Henan cohort has significantly higher prevalence (OR = 10.55, p = 0.0035). Results were independently validated in an ESCC Hong Kong cohort (OR = 10.64, p = 0.022). One Hong Kong pedigree was identified to carry a BRCA2 LOF mutation. BRCA2 inactivation in ESCC was via germline LOF mutations and wild‐type somatic allelic loss via loss of heterozygosity. Gene‐based association analysis, including LOF mutations and rare deleterious missense variants defined with combined annotation dependent depletion score ≥30, confirmed the genetic predisposition role of BRCA2 (OR = 9.50, p = 3.44 × 10 −5 ), and provided new evidence for potential association of ESCC risk with DNA repair genes ( POLQ and MSH2 ), inflammation ( TTC39B) and angiogenesis ( KDR ). Our findings are the first to provide compelling evidence of the role of BRCA2 in ESCC genetic susceptibility in Chinese, suggesting defective homologous recombination is an underlying cause in ESCC pathogenesis, which is amenable to therapeutic options based on synthetic lethality approaches such as targeting BRCA2 with PARP1 inhibitors in ESCC. Abstract : What's new? Esophageal cancer occurs at unusually high rates in Henan, China, and prognosis is poor. These authors set out to find genes associated with esophageal squamous cell carcinoma (ESCC) in this population. Based on whole exome sequencing, the researchers showed that loss of function mutations in BRCA2 increase ESCC risk. They also found evidence suggesting an association between ESCC and two other DNA repair genes, POLQ and MSH2 . This is the first study of familial ESCC in a genetically‐enriched cohort of patients from Henan. Screening for BRCA2 mutations may improve cancer detection and prognosis among patients with a family history of ESCC.