Multi-hydrolytic enzyme accumulation and microbial community structure of anaerobic co-digestion of food waste and waste-activated sludge. Issue 4 (10th February 2020)

• Record Type: Journal Article
• Authors: Luo, Kun; Xie, Xiao; Yang, Qi; Chen, Fei; Zhong, Yu; Xie, Pin; Wang, Gui
• Is Part Of: Environmental technology. Volume 41:Issue 4(2020)
• Page Start: 478
• Page End: 487
• Publication Date: 2020-02-10
• Subjects: Anaerobic co-digestion -- enzyme -- hydrolysis -- waste-activated sludge -- food waste; Environmental engineering -- Periodicals; Environmental protection -- Periodicals; 628.05
• DOI: 10.1080/09593330.2018.1502364
• Languages: English
• ISSNs: 0959-3330

Abstract

ABSTRACT: The accumulation of multi-hydrolytic enzyme through anaerobic co-digestion of waste-activated sludge (WAS) and food waste (FW) was studied by regulating temperature, pH and the mass ratio of FW to WAS (F/W). Experimental results showed that temperature had a profound effect on the activity of the enzyme and the most suitable temperatures for the accumulation of amylase and protease were 37°C and 50°C, respectively. The highest activity of amylase and protease accumulated reached 10.29 and 19.23 U/mL at an F/W ratio of 2:1. The addition of anaerobic co-digestion solution enriching protease and amylase had positive effects on the hydrolysis of WAS. In addition, the Illumina high-throughput sequencing demonstrated that the bacterial diversity decreased, but the bacterial abundance increased during the co-digestion process of WAS and FW. The predominant strains for secreting amylase were Lactobacillus and Clostridium-sensu-strito-1, and Aeromonas was the dominant strain for secreting protease. GRAPHICAL ABSTRACT: