Botrydial confers Botrytis cinerea the ability to antagonize soil and phyllospheric bacteria. Issue 1 (January 2020)

• Record Type: Journal Article
• Authors: Vignatti, Paulina; Gonzalez, María E.; Jofré, Edgardo C.; Bolívar-Anillo, Hernando J.; Moraga, Javier; Viaud, Muriel; Collado, Isidro G.; Pieckenstain, Fernando L.
• Is Part Of: Fungal biology. Volume 124:Issue 1(2020)
• Page Start: 54
• Page End: 64
• Publication Date: 2020-01
• Subjects: Antibacterial compound -- Bacillus spp. -- Biocontrol -- Cyclic lipopeptides -- Gray mold -- Phytotoxin; CLP cyclic lipopeptide -- TSA tryptic soy agar -- TSB tryptic soy broth; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2019.11.003
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: The role of the sesquiterpene botrydial in the interaction of the phytopathogenic fungus Botrytis cinerea and plant-associated bacteria was analyzed. From a collection of soil and phyllospheric bacteria, nine strains sensitive to growth-inhibition by B. cinerea were identified. B. cinerea mutants unable to produce botrydial caused no bacterial inhibition, thus demonstrating the inhibitory role of botrydial. A taxonomic analysis showed that these bacteria corresponded to different Bacillus species (six strains), Pseudomonas yamanorum (two strains) and Erwinia aphidicola (one strain). Inoculation of WT and botrydial non-producing mutants of B. cinerea along with B acillus amyloliquefaciens strain MEP2 18 in soil demonstrated that both microorganisms exert reciprocal inhibitory effects; the inhibition caused by B. cinerea being dependent on botrydial production. Moreover, botrydial production was modulated by the presence of B. amyloliquefaciens MEP2 18 in confrontation assays in vitro . Purified botrydial in turn, inhibited growth of Bacillus strains in vitro and cyclic lipopeptide (surfactin) production by B. amyloliquefaciens MEP2 18. As a whole, results demonstrate that botrydial confers B. cinerea the ability to inhibit potential biocontrol bacteria of the genus Bacillus . We propose that resistance to botrydial could be used as an additional criterion for the selection of biocontrol agents of plant diseases caused by B. cinerea . Graphical abstract: Image 1 Highlights: The sesquiterpene botrydial produced by Botrytis cinerea inhibits bacteria associated to plants. Botrydial lets B. cinerea antagonize Bacillus amyloliquefaciens in soil. Botrydial lets B. cinerea resist the antagonistic effects of B. amyloliquefaciens. Botrydial inhibits surfactin production by antagonistic B. amyloliquefaciens. The ecological role of botrydial is wider than its phytotoxic effects on plant hosts of B. cinerea.