Identification of Gene Transcripts Implicated in Peritoneal Membrane Alterations. Issue 6 (November 2016)

• Record Type: Journal Article
• Authors: Parikova, Alena; Vlijm, Anniek; Brabcova, Irena; de Graaff, Marijke; Struijk, Dirk G.; Viklicky, Ondrej; Krediet, Raymond T.
• Is Part Of: Peritoneal dialysis international. Volume 36:Issue 6(2016)
• Page Start: 606
• Page End: 613
• Publication Date: 2016-11
• Subjects: Peritoneal dialysis -- peritoneal membrane alterations -- peritoneal transport -- fibrosis -- angiogenesis -- inflammation -- gene expression; Peritoneal dialysis -- Periodicals; Continuous ambulatory peritoneal dialysis -- Periodicals; 617.461059
• DOI: 10.3747/pdi.2015.00094
• Languages: English
• ISSNs: 0896-8608

Abstract

Background: Permanent stimulation of the peritoneum during peritoneal dialysis (PD) is likely to result in increased expression of genes encoding proteins involved in inflammation and tissue remodeling. Peritoneal fibrosis and neoangiogenesis may develop. Objective: To assess highly expressed genes potentially involved in peritoneal alterations during PD treatment using an animal model. Methods: A PD catheter was implanted in 36 male Wistar rats after 70% nephrectomy. The rats were divided into 3 groups, exposed to dialysis solution for 8 weeks, and sacrificed 2 weeks later. Group B was exposed to a buffer, group D was exposed to a 3.86% glucose-based dialysis solution, and in group D+H, a second hit of intraperitoneal blood on top of the dialysis solution was given to induce the development of peritoneal sclerosis. Before sacrifice, peritoneal function was assessed. Omental tissue was obtained for analysis of gene expression using RT-qPCR. Results: Fibrosis scores, vessel counts, and peritoneal function parameters were not different between the groups. Genes involved in the transforming growth factor beta signaling pathway, cell proliferation, angiogenesis, and inflammation were more expressed ( p < 0.05) in the D+H group. Almost no differences were found between the control groups. We identified 4 genes that were related to peritoneal transport. Conclusion: Already a mid-term peritoneal exposure, when no microscopical and functional alterations are present, provokes activation of gene pathways of cell proliferation, fibrosis, neoangiogenesis, and inflammation.