Isolation and comparison of α- and β-D-glucans from shiitake mushrooms (Lentinula edodes) with different biological activities. (1st February 2020)

• Record Type: Journal Article
• Authors: Morales, Diego; Rutckeviski, Renata; Villalva, Marisol; Abreu, Hellen; Soler-Rivas, Cristina; Santoyo, Susana; Iacomini, Marcello; Smiderle, Fhernanda Ribeiro
• Is Part Of: Carbohydrate polymers. Volume 229(2020)
• Publication Date: 2020-02-01
• Subjects: G-1 Glucan-I -- G-2 Glucan-II -- G-3 Glucan-III -- HMGCR 3-hydroxy-3-methylglutaryl coenzyme A reductase; β-Glucans -- α-Glucans -- Shiitake mushroom -- Hypocholesterolemic -- Anti-inflammatory -- Cytotoxic; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115521
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: Three purified glucan fractions were obtained from shiitake mushrooms. A branched (1→3), (1→6)-β-D-glucan and a linear (1→3)-α-D-glucan were isolated. A linear (1→6)-β-D-glucan-enriched fraction was also obtained. Glucans showed in vitro hypocholesterolemic and immunomodulatory activities. The main differences were observed on in vitro antioxidant and antitumoral activity. Abstract: A polysaccharide-enriched extract obtained from Lentinula edodes was submitted to several purification steps to separate three different D-glucans with β-(1→6), β-(1→3), (1→6) and α-(1→3) linkages, being characterized through GC–MS, FT-IR, NMR, SEC and colorimetric/fluorimetric determinations. Moreover, in vitro hypocholesterolemic, antitumoral, anti-inflammatory and antioxidant activities were also tested. Isolated glucans exerted HMGCR inhibitory activity, but only β-(1→6) and β-(1→3), (1→6) fractions showed DPPH scavenging capacity. Glucans were also able to lower IL-1β and IL-6 secretion by LPS-activated THP-1/M cells and showed cytotoxic effect on a breast cancer cell line that was not observed on normal breast cells. These in vitro results pointed important directions for further in vivo studies, showing different effects of each chemical structure of the isolated glucans from shiitake mushrooms.