Preparation and characterization of chemically TEMPO-oxidized and mechanically disintegrated sacchachitin nanofibers (SCNF) for enhanced diabetic wound healing. (1st February 2020)

• Record Type: Journal Article
• Authors: Chao, Fang-Ching; Wu, Meng-Huang; Chen, Ling-Chun; Lin, Hong-Liang; Liu, Der-Zen; Ho, Hsiu-O; Sheu, Ming-Thau
• Is Part Of: Carbohydrate polymers. Volume 229(2020)
• Publication Date: 2020-02-01
• Subjects: 2, 2, 6, 6-tetramethyl-1-piperidinyloxy (TEMPO) -- 2-acrylamido-2-methyl-1-propanesulfonic acid sodium salt (AMPS) -- Chitosan -- PEG1000 -- PVP K90 -- L-ascorbic acid, poly(ethylene glycol) diacrylate -- N, N-dimethylacrylamide -- Sodium hypochlorite -- Sodium bromide -- Thiazolyl blue tetrazolium bromide (MTT reagent) -- Irgacure® 184; Sacchachitin -- TEMPO-oxidation -- Mechanical disintegration -- Nanofibers -- Diabetic wound healing; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2019.115507
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: TEMPO-oxidized SCNF (TOSCNF) and microfludized SCNF (MDSCNF) were prepared and evalued. All 2% MDSCNF suspension were demonstrated to be in a gel form. All except T100SC of 2% TOSCNF suspension showed to be wet fiber-like hydrogel. SCN5/H and T050SC/H accelerated diabetic wound healing to similar as normal tissue. T050SC/H provided the healed wound as functional tissue instead of scar tissue. Abstract: TEMPO-oxidization and mechanical disintegration were utilized to develop sacchachitin nanofibers (SCNF) with a 3D gel structure for being an ideal scaffold. Mechanically disintegrated SCNF (MDSCNF) with NanoLyzer® at 20, 000 psi for 5 cycles and TEMPO-oxidized SCNF (TOSCNF) produced with 5.0 and 10.0 mmole NaClO/g SC was designated as SCN5, T050SC, and T100SC, respectively. All 2% MDSCNF suspensions were demonstrated to be in gel form, while all except T100SC of 2% TOSCNF suspensions showed to be wet fiber-like hydrogel. In diabetic wound healing study, both SCN5 and T050SC incorporated in AMPS (2-acrylamide-2-methyl-propane sulfonate)-based wound dressing were showed to accelerate diabetic wound healing forming nearly the same as normal tissues. T050SC/H further provided the healed wound with growth of sweat glands and hair follicles indicating the wound had healed as functional tissue. Conclusively, TEMPO-oxidized SCNF-based hydrogel scaffolds showed greater potentials in tissue regeneration due to its unique physical and chemical properties.