NMR Perspectives of the KcsA Potassium Channel in the Membrane Environment. Issue 11 (15th November 2019)

• Record Type: Journal Article
• Authors: Chill, Jordan H.; Qasim, Arwa; Sher, Inbal; Gross, Renana
• Is Part Of: Israel journal of chemistry. Volume 59:Issue 11/12(2019)
• Page Start: 1001
• Page End: 1013
• Publication Date: 2019-11-15
• Subjects: Membrane proteins -- KcsA channel -- Lipoprotein nanodiscs -- Nuclear magnetic resonance -- Electron paramagnetic resonance; Chemistry -- Periodicals; 540.5
• DOI: 10.1002/ijch.201900107
• Languages: English
• ISSNs: 0021-2148

Abstract

Abstract: Membrane‐embedded proteins (MPs) are central to a wide range of cellular processes. Despite their importance, structural studies of MPs are hindered by expression difficulties and the need for stabilization in a membrane‐mimicking environment. High‐resolution NMR methods can investigate structure and function of MPs due to methodological advances and new membrane‐like assemblies for stabilization of MPs. In this perspective of the field, we introduce the challenges and opportunities of NMR studies of membrane proteins, briefly surveying membrane‐mimicking systems and their application in structure determination. A case study then focuses on the C‐terminal domain of the bacterial potassium channel KcsA, describing how improvements in membrane‐mimicking conditions eventually enabled us to present a structural view of the pH‐dependent behavior of this cytoplasmic channel domain. The results highlight prerequisites for a successful study of MPs and the potential for future investigations. Abstract :