Lippia sidoides and Lippia origanoides essential oils affect the viability, motility and ultrastructure of Trypanosoma cruzi. (February 2020)

• Record Type: Journal Article
• Authors: de Melo, Andrezza Raposo Borges; Maciel Higino, Taciana Mirely; da Rocha Oliveira, Aline Dulce Pitt; Fontes, Adriana; da Silva, Diego César Nunes; de Castro, Maria Carolina Accioly Brelaz; Dantas Lopes, José Arimatéia; de Figueiredo, Regina Celia Bressan Queiroz
• Is Part Of: Micron. Volume 129(2020)
• Publication Date: 2020-02
• Subjects: Essential oils -- Trypanosoma cruzi -- Lippia sidoides -- Lippia origanoides -- Ultrastructure -- Optical tweezer -- Chemotherapy; Microscopy -- Periodicals; Electron Probe Microanalysis -- Periodicals; Microscopy -- Periodicals; Microscopie -- Périodiques; Microscopy; Periodicals; 502.82
• DOI: 10.1016/j.micron.2019.102781
• Languages: English
• ISSNs: 0968-4328

Abstract

Graphical abstract: Highlights: Essential oils from Lippia sidoides and L. origanoides reduced the infection of macrophages by T. cruzi . Essential Oils from L. sidoides and L. origanoides caused decrease in the cell viability and loss of membrane integrity on T. cruzi . The ultrastructural changes points the parasite mitochondrion as target of Essential Oils action. Optical tweezers can be a useful tool to investigate the impact of a drug treatment on the motility of parasites. Abstract: Chagas disease, caused by the protozoan Trypanosoma cruzi, is considered a public health problem. The current chemotherapy for this illness causes serious side effects and its use in the chronic phase of the disease is still controversial. In this regard, the investigation of novel therapeutic strategies remains a priority. The essential oils (EOs) from aromatic plants emerge as a promising source of bioactive compounds. In a previous work we reported the trypanocidal activity of the essential oils from the medicinal plants Lippia sidoides (LSEO) and Lippia origanoides (LOEO) against T. cruzi . Herein, we aimed to further investigate, in more details, the mode of action of LSEO and LOEO on the different developmental stages of this parasite. We showed that Lippia sidoides (LSEO) and Lippia origanoides (LOEO) induced a significant reduction in the percentage of macrophages infected by T. cruzi and in the number of intracellular parasites. Ultrastructural analysis showed that the treatment with both oils caused morphological changes consistent with loss of viability and cell death. The reduced staining with calcein and the increase in the proportion of HE–positive cells also demonstrated that LSEO and LOEO caused loss of parasite viability and membrane integrity. A considerable decrease in Rhodamine 123 and an increase in fluorescence intensity of MitoSox in LOEO were indicative of loss of mitochondrial potential and generation of reactive oxygen species, which ultimately lead to parasite death. Moreover, the optical tweezer analysis indicated that LOEO was more effective in reducing the motility of the epimastigotes. Taken together, our results demonstrated that the LSEO and LOEO are active against T. cruzi and constitute a promising drugs for the therapy of Chagas disease.