Polymorphisms of CHRNA3 and CHRNA5: Head and neck cancer and cigarette consumption intensity in a Brazilian population. Issue 12 (9th October 2019)

• Record Type: Journal Article
• Authors: Silva, Mariana R.; Gattás, Gilka J. F.; De Antonio, Juliana; Firigato, Isabela; Curioni, Otávio A.; Gonçalves, Fernanda de Toledo
• Is Part Of: Molecular genetics & genomic medicine. Volume 7:Issue 12(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-10-09
• Subjects: CHRNA3 -- CHRNA5 -- cigarette consumption -- head and neck cancer; Medical genetics -- Periodicals; Genomics -- Periodicals; 616.042
• DOI: 10.1002/mgg3.998
• Languages: English
• ISSNs: 2324-9269

Abstract

Abstract: Background: Cigarette consumption has been identified as the main non‐etiological factor in head and neck cancer (HNC) development. One of the main compounds in cigarettes is nicotine, which binds directly to nicotine acetylcholine receptors (nAchRs) in the body, which are encoded by different genes of the CHRNA family. Polymorphisms in some of these genes have been studied in relation to the risk of HNC and cigarette consumption intensity. The aim of this study was to evaluate whether there were associations between the CHRNA3 (rs578776) and CHRNA5 (rs16969968) polymorphisms and HNC risk and between the polymorphisms and the intensity of cigarette consumption. Methods: A total of 1, 067 individuals from Heliopolis Hospital in São Paulo were investigated, including 619 patients with HNC and 448 patients without diagnosed tumors. All participants answered a questionnaire about sociodemographic information and cigarette consumption data. The polymorphisms were determined by TaqMan genotyping by real‐time PCR. Results: The polymorphisms studied, rs578776 ( CHRNA3 ) and rs16969968 ( CHRNA5 ), did not have an association with HNC risk, but the rs16969968 homozygous genotype was associated with increased cigarette consumption intensity (OR 1.93, 95% CI 1.05–3.58). Conclusion: The polymorphism CHRNA5 can be considered an indirect risk factor for neoplasms in these Brazilian samples when cigarette consumption increased. Abstract : The gene CHRNA5, that encoded the α5 subunit of nicotinic receptor, increased the cigarette consumption intensity in Brazilian sample. Once tobacco consumed is a risk factor to the head and neck cancer development, these polymorphism can be considered an indirect risk factor for these neoplasm.