Change in phospholipid species of retinal layer in traumatic optic neuropathy model. Issue 2 (5th August 2019)

• Record Type: Journal Article
• Authors: Hirahara, Yukie; Wakabayashi, Taketoshi; Koike, Taro; Gamo, Keizo; Yamada, Hisao
• Other Names: Martín‐Segura Adrián guestEditor.; Benvegnù Stefano guestEditor.
• Is Part Of: Journal of neuroscience research. Volume 98:Issue 2(2020)
• Page Start: 325
• Page End: 337
• Publication Date: 2019-08-05
• Subjects: imaging mass spectrometry -- phosphatidyletanolamine -- phosphatidylinositol -- phosphatidylserine -- retina -- RRID:AB_1004487 -- RRID:AB_477035 -- RRID:AB_477257 -- traumatic optic nerve injury; Neurobiology -- Periodicals; 612
• DOI: 10.1002/jnr.24500
• Languages: English
• ISSNs: 0360-4012

Abstract

Abstract: Injured optic nerves induce death in almost all retinal ganglion cells (RGC) and cause a loss of axons. To date, we have studied injured RGC axon regeneration by using a traumatic optic nerve injury (TONI) rodent model, and we revealed that axonal regeneration is induced by the graft of an autologous peripheral nerve. The efficient approach to the regeneration of axons thus needs an environmental adjustment of RGC. However, the RGC environment induced by TONI remains unknown. Here, we analyzed female and male C57BL/6 mouse retinal tissue alterations in detail after TONI and focused on the major phospholipid species that are enriched in the whole retina. Reactive astrocyte accumulation, glia scar formation, and demyelination were observed in the injured optic nerve area, while RGC cell death, astrocyte accumulation, and Glial fibrillary acidic protein (GFAP) positive Müller cell increases were detected in the retinal layer. Furthermore, phosphatidylinositol (PI) 18:0/20:4 was localized to three nuclear layer structures: the ganglion cell layer (GCL), the inner nuclear layer (INL), and the outer nuclear layer (ONL) in control retina; however, the localization of 18:0/20:4 PI in TONI was disturbed. Meanwhile, phosphatidylserine (PS) 18:0/22:6 showed that the expression was specifically in the inner plexiform layer (IPL) with similar signal intensity in both cases. Other PS species and phosphatidylethanolamine (PE) were differentially localized in the retinal layer; however, the expressions of PE including docosahexaenoic acid (DHA) were affected by TONI. These results suggest that not only GCL but also other retinal layers were influenced by TONI. Abstract : To understand the retinal environmental changes by injured optic nerves, we focused on the major phospholipid species that are enriched in the whole retina. Here, we show the spatial distributions of phospholipid species and the effect by injured optic nerves in mouse retina by using imaging mass spectrometry.