A fluorescent probe for butyrylcholinesterase activity in human serum based on a fluorophore with specific binding affinity for human serum albumin. Issue 97 (30th October 2019)

• Record Type: Journal Article
• Authors: Yoo, Soyeon; Han, Min Su
• Is Part Of: Chemical communications. Volume 55:Issue 97(2019)
• Page Start: 14574
• Page End: 14577
• Publication Date: 2019-10-30
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc07737e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We report a novel turn-on sensing probe for the detection of butyrylcholinesterase activity in human serum using a fluorophore with high binding affinity for HSA. Abstract : Non-specific binding of a fluorescent probe to human serum albumin is problematic because it induces signal interference when the probe detects the target biomarker in human serum. To eliminate this problem, we used intrinsically problematic non-specific fluorescence in designing a fluorescent probe for butyrylcholinesterase activity in serum. The probe containing a fluorophore with specific binding affinity for albumin could sensitively detect butyrylcholinesterase activity in serum with high selectivity to acetylcholinesterase and screen the efficiency of butyrylcholinesterase inhibitors.