Chemo-biological mRNA imaging with single nucleotide specificity. Issue 98 (25th November 2019)

• Record Type: Journal Article
• Authors: Knoll, Andrea; Kankowski, Svenja; Schöllkopf, Sophie; Meier, Jochen C.; Seitz, Oliver
• Is Part Of: Chemical communications. Volume 55:Issue 98(2019)
• Page Start: 14817
• Page End: 14820
• Publication Date: 2019-11-25
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c9cc06989e
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : The combined use of "biological" RNA imaging methods based on MS2 technology and "chemical" RNA detection by FIT probes allows unambiguous cellular imaging of a C → U edit in mRNA encoding for GlyR α2. Abstract : Unambiguous imaging of C → U edited mRNA calls for a method that distinguishes a locally high concentration of unbound probe or single nucleotide mismatched target from a locally low concentration of matched mRNA target. To address this issue, we combine FIT probes as a "chemical" detection system with the "biological" MS2 technique. Ratio measurements provide a convenient parameter to discriminate the edited from the unedited state of mRNA encoding for GlyR α2 in HEK cells.