Prevalence of colistin resistance and mcr-1/mcr-2 genes in extended-spectrum β-lactamase/AmpC-producing Escherichia coli isolated from chickens in Canada, Senegal and Vietnam. (December 2019)

• Record Type: Journal Article
• Authors: Vounba, Passoret; Rhouma, Mohamed; Arsenault, Julie; Bada Alambédji, Rianatou; Fravalo, Philippe; Fairbrother, John Morris
• Is Part Of: Journal of global antimicrobial resistance. Volume 19(2019)
• Page Start: 222
• Page End: 227
• Publication Date: 2019-12
• Subjects: Chickens -- Colistin -- Escherichia coli -- Extended-spectrum β-lactamase -- mcr -- Prevalence; Drug resistance -- Periodicals; Drug resistance -- Periodicals; Drug resistance; Periodicals; 616.9041
• DOI: 10.1016/j.jgar.2019.05.002
• Languages: English
• ISSNs: 2213-7165

Abstract

Highlights: No E. coli isolates from Quebec poultry farms were colistin-resistant or possessed mcr (1-2) genes. Two E. coli isolates from Senegal poultry farms were colistin-resistant but mcr (1-2) negative. New mutation in the PmrAB of colistin resistant E. coli isolates was detected. Eleven E. coli isolates from Vietnam poultry farms were colistin-resistant and mcr-1 positive. All colistin resistant, ESBL/AmpC producing E. coli isolates were highly multidrug resistant. Abstract: Objectives: This study investigated the prevalence of Escherichia coli ( E. coli ) colistin resistance and mcr-1 and mcr-2 genes among extended-spectrum β-lactamase (ESBL)/AmpC-producing E. coli isolates recovered from chicken feces in Canada (Quebec), Senegal and Vietnam, and evaluated the susceptibility pattern of the colistin-resistant E. coli isolates to other clinically relevant antimicrobials. Methods: A total of 327 potential ESBL/AmpC-producing E. coli isolates from chicken farms in Canada (Quebec), Senegal and Vietnam were analysed for colistin susceptibility by broth microdilution method and for the presence of mcr ( 1 - 2 ) genes by PCR. The pmrA and pmrB genes of colistin-resistant E. coli isolates, in the absence of mcr ( 1-2 ) genes, were sequenced. Antimicrobial resistance phenotypes of colistin-resistant E. coli isolates were determined by disk diffusion. Results: None of the 108 potential ESBL/AmpC-producing E. coli isolates from seven farms in Canada were colistin-resistant or possessed mcr - 1 or mcr-2 gene. A low prevalence of 2.2% of colistin resistance was observed in 93 Senegalese isolates from the 15 sampled farms, although neither mcr - 1 nor mcr-2 gene was found. A prevalence of 8.7% of colistin resistance was observed among 126 Vietnamese isolates from two of the four sampled farms. The mcr-1 gene was detected in 85% of the 13 phenotypically colistin-resistant isolates. Moreover, all colistin-resistant isolates presented a multidrug-resistant phenotype. Conclusions: The co-existence of the mcr-1 and ESBL/AmpC genes and the very high level of multiple drug resistance in all colistin-resistant E. coli isolates obtained from sampled chicken farms in Vietnam is a major concern.