Microbial community response to ammonia levels in hydrogen assisted biogas production and upgrading process. (January 2020)

• Record Type: Journal Article
• Authors: Wang, Han; Zhu, Xinyu; Yan, Qun; Zhang, Yifeng; Angelidaki, Irini
• Is Part Of: Bioresource technology. Volume 296(2020)
• Publication Date: 2020-01
• Subjects: Anaerobic digestion -- Biogas upgrading -- Microbial community -- Ammonia inhibition -- Hydrogenotrophic methanogens; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2019.122276
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A change to hydrogenotrophic pathway occurred when ammonia increased. Bacteria which were vulnerable to ammonia could be restored by hydrogen injection. Both archaea and bacteria's tolerance to ammonia were improved by adding hydrogen. Higher abundances of archaea OTUs in thermophilic condition were detected. Abstract: Biological conversion of carbon dioxide into methane using hydrogen derived from surplus renewable energy (wind power) as reducing power is a novel technology for biogas upgrading. High ammonia concentrations are toxic to the biogas upgrading process, however the mechanisms behind the inhibition as well as the microbial stress response in such unique upgrading system have never been reported. Thus, the effect of high ammonia concentrations on microbial community during hydrogen induced biogas upgrading process was evaluated here. The results showed that a change from aceticlastic pathway to hydrogenotrophic pathway occurred when ammonia level increased (1–7 g NH4 + -N L −1 ). In addition, the bacteria, potentially syntrophic associated with hydrogenotrophic methanogens, were enriched at high ammonia concentrations. Moreover, growth of some bacteria (e.g., Halanaerobiaceaeen and Leucobacter ) which were vulnerable to ammonia toxicity was restored upon hydrogen injection. Furthermore, hydrogen injection under high ammonia concentration could promote growth of some hydrolytic and fermentative bacteria.