Dansyl-peptide dual-functional fluorescent chemosensor for Hg2+ and biothiols. (February 2020)

• Record Type: Journal Article
• Authors: Pang, Xuliang; Dong, Jianfang; Gao, Lei; Wang, Lei; Yu, Shuaibing; Kong, Jinming; Li, Lianzhi
• Is Part Of: Dyes and pigments. Volume 173(2020)
• Publication Date: 2020-02
• Subjects: Peptide chemosensor -- FRET -- Mercury ion -- Biothiols; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2019.107888
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A new dansyl-peptide dual-functional fluorescent chemosensor (D-ECEW-NH2, D-P4) was developed via Fmoc solid phase peptide synthesis (SPPS), which displayed an excellent selectivity and high sensitivity for Hg 2+ by turn on response in 10 mM 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES) solution (pH 7.1). The sensing mechanism for Hg 2+ is by two pathways, one is fluorescence resonance energy transfer (FRET) effect between tryptophan (donor) and dansyl (acceptor) fluorophores due to the interaction of Hg 2+ ion with D-P4 evidenced by fluorescence excitation spectra at 290 nm, while the other is the chelation enhanced fluorescence (CHEF) effect evidenced by fluorescence excitation spectra at 330 nm. D-P4 sensor was used to detect Hg 2+ with low detection limit of 23.0 nM. Furthermore, the binding stoichiometry and binding constant, as well as pH influence of D-P4 for Hg 2+ were investigated. Surprising but interestingly, D-P4-Hg system has been found a significant fluorescence enhancement response toward biothiols including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), which can be used for the detection of biothiols. Its detection limit for Cys was 52.0 nM. Graphical abstract: A simple peptidyl dual-functional fluorescent chemosensor (Dansyl-Glu-Cys-Glu-Trp-NH2, D-P4) has been developed for highly selective and sensitive detection of Hg 2+ over other common metal ions by fluorescence resonance energy transfer (FRET) effect and chelation enhanced fluorescence (CHEF) effect, and D-P4-Hg 2+ complex was used as an excellent fluorescent sensor for biothiols (−SH) detection. Image 1 Highlights: A new dansyl-peptide dual-functional fluorescent chemosensor was synthesized and characterized. The sensor displayed an excellent selectivity and high sensitivity for Hg 2+ . The sensing mechanism for Hg 2+ is via FRET effect and CHEF effect. The complex of sensor with Hg(II) can be developed a sensitive sensor for the detection of biothiols.