Differential expression of transcripts with potential relevance to chlorantraniliprole response in the Colorado potato beetle, Leptinotarsa decemlineata. Issue 1 (30th October 2019)

• Record Type: Journal Article
• Authors: Dumas, Pascal; Sambou, Mariama; Gaudet, Josée D.; Morin, Mathieu D.; Moffat, Chandra E.; Boquel, Sébastien; Jr Morin, Pier
• Is Part Of: Archives of insect biochemistry and physiology. Volume 103:Issue 1(2020)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2019-10-30
• Subjects: chlorantraniliprole -- Colorado potato beetles -- cytochrome P450s -- microRNAs -- noncoding RNAs; Insects -- Physiology -- Periodicals; Insect biochemistry -- Periodicals; 595.701572
• DOI: 10.1002/arch.21642
• Languages: English
• ISSNs: 0739-4462

Abstract

Abstract: The Colorado potato beetle ( Leptinotarsa decemlineata [Say]) is an insect pest that can significantly harm potato plants worldwide. Control of this insect relies heavily on chemical insecticides such as chlorantraniliprole. Nevertheless, the complete molecular signature associated with response to this compound is lacking in L. decemlineata . In this study, amplification and quantification by qRT‐PCR (quantitative reverse transcription‐polymerase chain reaction) of targets relevant to chlorantraniliprole were undertaken in insects exposed to this chemical. This approach showed modulation of numerous cytochrome P450s, such as CYP350D1 and CYP4Q3, as well as upregulation of microRNAs (miRNAs), including miR‐1‐3p and miR‐305‐5p, in chlorantraniliprole‐exposed insects. Functional assessment of transcript targets predicted to be regulated by these miRNAs was performed and revealed their likely impact on transcriptional regulation. RNAi‐based targeting of CYP350D1 notably provided preliminary evidence of its underlying implication for chlorantraniliprole response in L. decemlineata . Overall, this study strengthens the current knowledge of the molecular changes linked to chlorantraniliprole response in L. decemlineata and provides novel targets with potential relevance to chlorantraniliprole susceptibility in this insect pest of global relevance. Abstract : Differential expression of select transcript targets with relevance to chlorantraniliprole response in Colorado potato beetles. Research Highlights: Quantitative reverse transcription‐polymerase chain reaction approach undertaken in chlorantraniliprole‐exposed Leptinotarsa decemlineata Modulation of cytochrome P450s transcripts in insects treated with chlorantraniliprole Upregulation of select miRNAs with relevance to insecticides response