More precise, more universal and more specific – the next generation of RNA‐guided endonucleases for genome editing. (14th October 2019)

• Record Type: Journal Article
• Authors: Marzec, Marek; Hensel, Goetz
• Is Part Of: FEBS journal. Volume 286:Number 23(2019)
• Page Start: 4657
• Page End: 4660
• Publication Date: 2019-10-14
• Subjects: Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pathology, Molecular -- Periodicals; 572
• DOI: 10.1111/febs.15079
• Languages: English
• ISSNs: 1742-464X

Abstract

Abstract : CRISPR is a prokaryotic defence system that was adapted as a tool for genome editing and has become one of the most important discoveries of this century. CRISPR‐associated endonucleases cleave DNA at precise sites, which are marked by complementary short‐guided RNA. The recently developed versions of endonucleases are compatible with a broad range of PAM motifs, have a higher specificity and enable a specific nucleotide to be replaced.