Effects of anticoagulants on stable‐isotope values (δ13C and δ15N) of shark blood components. Issue 6 (6th November 2019)

• Record Type: Journal Article
• Authors: Weideli, Ornella C.; Kiszka, Jeremy J.; Matich, Philip; Heithaus, Michael R.
• Is Part Of: Journal of fish biology. Volume 95:Issue 6(2019)
• Page Start: 1535
• Page End: 1539
• Publication Date: 2019-11-06
• Subjects: blood plasma -- blood preservatives -- Carcharhinus melanopterus -- δ13C -- δ15N -- red blood cells (RBC); Fishes -- Periodicals; Fishes -- Great Britain -- Periodicals; 597
• DOI: 10.1111/jfb.14164
• Languages: English
• ISSNs: 0022-1112

Abstract

Abstract: The effects of anticoagulant EDTA and sodium heparin (SH) on stable carbon δ 13 C and nitrogen δ 15 N isotopic values of red blood cells (RBC) and blood plasma in juvenile blacktip reef sharks Carcharhinus melanopterus were analysed. Plasma preserved with anticoagulants was not isotopically distinct from plasma stored in no‐additive control tubes but RBC δ 15 N values exhibited small enrichments when preserved with EDTA and SH. Results suggest EDTA and SH are viable anticoagulants for stable isotopic analyses of blood fractions but further studies are advised to validate results.