Anti-OspA DNA-Encoded Monoclonal Antibody Prevents Transmission of Spirochetes in Tick Challenge Providing Sterilizing Immunity in Mice. (21st November 2018)

• Record Type: Journal Article
• Authors: Wang, Yang; Esquivel, Rianne; Flingai, Seleeke; Schiller, Zachary A; Kern, Aurélie; Agarwal, Sangya; Chu, Jacqueline; Patel, Ami; Sullivan, Katherine; Wise, Megan C; Broderick, Kate E; Hu, Linden; Weiner, David B; Klempner, Mark S
• Is Part Of: Journal of infectious diseases. Volume 219:Number 7(2019)
• Page Start: 1146
• Page End: 1150
• Publication Date: 2018-11-21
• Subjects: Lyme disease -- DMAb -- OspA -- human monoclonal antibody; Communicable diseases -- Periodicals; Diseases -- Causes and theories of causation -- Periodicals; Medicine -- Periodicals; Communicable Diseases -- Periodicals; Electronic journals; 616.9
• DOI: 10.1093/infdis/jiy627
• Languages: English
• ISSNs: 0022-1899

Abstract

Abstract: We recently developed anti-OspA human immunoglobulin G1 monoclonal antibodies (HuMAbs) that are effective in preventing Borrelia transmission from ticks in a murine model. Here, we investigated a novel approach of DNA-mediated gene transfer of HuMAbs that provide protection against Lyme disease. Plasmid DNA-encoded anti-OspA HuMAbs inoculated in mice achieved a serum antibody concentration of >6 μg/mL. Among mice injected with DNA-encoded monoclonal antibodies, 75%–77% were protected against an acute challenge by Borrelia -infected ticks. Our results represent the first demonstration of employing DNA transfer as a delivery system for antibodies that block transmission of Borrelia in animal models. Abstract : We investigated a novel approach of DNA-mediated gene transfer of anti-OspA human immunoglobulin G1 monoclonal antibodies that provide protection against Lyme disease. Our results represent the first demonstration of employing DNA transfer to produce antibodies that block transmission by Borrelia -infected ticks.