Cytokinin Signaling Is Essential for Organ Formation in Marchantia polymorpha. (28th May 2019)
- Record Type:
- Journal Article
- Title:
- Cytokinin Signaling Is Essential for Organ Formation in Marchantia polymorpha. (28th May 2019)
- Main Title:
- Cytokinin Signaling Is Essential for Organ Formation in Marchantia polymorpha
- Authors:
- Aki, Shiori S
Mikami, Tatsuya
Naramoto, Satoshi
Nishihama, Ryuichi
Ishizaki, Kimitsune
Kojima, Mikiko
Takebayashi, Yumiko
Sakakibara, Hitoshi
Kyozuka, Junko
Kohchi, Takayuki
Umeda, Masaaki - Abstract:
- Abstract: Cytokinins are known to regulate various physiological events in plants. Cytokinin signaling is mediated by the phosphorelay system, one of the most ancient mechanisms controlling hormonal pathways in plants. The liverwort Marchantia polymorpha possesses all components necessary for cytokinin signaling; however, whether they respond to cytokinins and how the signaling is fine-tuned remain largely unknown . Here, we report cytokinin function in Marchantia development and organ formation. Our measurement of cytokinin species revealed that cis -zeatin is the most abundant cytokinin in Marchantia . We reduced the endogenous cytokinin level by overexpressing the gene for cytokinin oxidase, Mp CKX, which inactivates cytokinins, and generated overexpression and knockout lines for type-A (Mp RRA ) and type-B (Mp RRB ) response regulators to manipulate the signaling. The overexpression lines of Mp CKX and Mp RRA, and the knockout lines of Mp RRB, shared phenotypes such as inhibition of gemma cup formation, enhanced rhizoid formation and hyponastic thallus growth. Conversely, the knockout lines of Mp RRA produced more gemma cups and exhibited epinastic thallus growth. Mp RRA expression was elevated by cytokinin treatment and reduced by knocking out Mp RRB, suggesting that Mp RRA is upregulated by the MpRRB-mediated cytokinin signaling, which is antagonized by MpRRA. Our findings indicate that when plants moved onto land they already deployed the negative feedback loop ofAbstract: Cytokinins are known to regulate various physiological events in plants. Cytokinin signaling is mediated by the phosphorelay system, one of the most ancient mechanisms controlling hormonal pathways in plants. The liverwort Marchantia polymorpha possesses all components necessary for cytokinin signaling; however, whether they respond to cytokinins and how the signaling is fine-tuned remain largely unknown . Here, we report cytokinin function in Marchantia development and organ formation. Our measurement of cytokinin species revealed that cis -zeatin is the most abundant cytokinin in Marchantia . We reduced the endogenous cytokinin level by overexpressing the gene for cytokinin oxidase, Mp CKX, which inactivates cytokinins, and generated overexpression and knockout lines for type-A (Mp RRA ) and type-B (Mp RRB ) response regulators to manipulate the signaling. The overexpression lines of Mp CKX and Mp RRA, and the knockout lines of Mp RRB, shared phenotypes such as inhibition of gemma cup formation, enhanced rhizoid formation and hyponastic thallus growth. Conversely, the knockout lines of Mp RRA produced more gemma cups and exhibited epinastic thallus growth. Mp RRA expression was elevated by cytokinin treatment and reduced by knocking out Mp RRB, suggesting that Mp RRA is upregulated by the MpRRB-mediated cytokinin signaling, which is antagonized by MpRRA. Our findings indicate that when plants moved onto land they already deployed the negative feedback loop of cytokinin signaling, which has an indispensable role in organogenesis. … (more)
- Is Part Of:
- Plant & cell physiology. Volume 60:Number 8(2019)
- Journal:
- Plant & cell physiology
- Issue:
- Volume 60:Number 8(2019)
- Issue Display:
- Volume 60, Issue 8 (2019)
- Year:
- 2019
- Volume:
- 60
- Issue:
- 8
- Issue Sort Value:
- 2019-0060-0008-0000
- Page Start:
- 1842
- Page End:
- 1854
- Publication Date:
- 2019-05-28
- Subjects:
- Cytokinin -- Marchantia polymorpha -- Organogenesis -- Plant evolution -- Response regulator
Plant physiology -- Periodicals
Microbiology -- Periodicals
Cytology -- Periodicals
Cell Physiology -- Periodicals
Plant Physiological Phenomena -- Periodicals
Cytology
Microbiology
Plant physiology
Periodicals
571.205 - Journal URLs:
- http://pcp.oupjournals.org/ ↗
http://pcp.oxfordjournals.org/ ↗
http://ukcatalogue.oup.com/ ↗
http://firstsearch.oclc.org ↗
http://firstsearch.oclc.org/journal=0032-0781;screen=info;ECOIP ↗ - DOI:
- 10.1093/pcp/pcz100 ↗
- Languages:
- English
- ISSNs:
- 0032-0781
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 6512.250000
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