Characterisation of the diversity and physiology of cellobiose-fermenting yeasts isolated from rotting wood in Brazilian ecosystems. Issue 7 (July 2018)

• Record Type: Journal Article
• Authors: Lopes, Mariana R.; Lara, Carla A.; Moura, Marina E.F.; Uetanabaro, Ana Paula T.; Morais, Paula B.; Vital, Marcos J.S.; Rosa, Carlos A.
• Is Part Of: Fungal biology. Volume 122:Issue 7(2018)
• Page Start: 668
• Page End: 676
• Publication Date: 2018-07
• Subjects: β-glucosidase -- Cellobiose fermentation -- Lignocellulosic ethanol -- Yeasts; Mycology -- Periodicals; Fungi -- Periodicals; 579.505
• DOI: 10.1016/j.funbio.2018.03.008
• Languages: English
• ISSNs: 1878-6146

Abstract

Abstract: We investigated the yeast species associated with rotting wood samples obtained from Brazilian ecosystems, with a special focus on cellobiose-fermenting species. About 647 yeast strains were isolated from rotting wood samples collected from the areas of Atlantic rainforest, Cerrado, and Amazonian forest. Eighty-six known species and 47 novel species of yeasts were isolated. Candida boidinii, Cyberlindnera subsufficiens, Meyerozyma guilliermondii, Schwanniomyces polymorphus, Candida natalensis, and Debaryomyces hansenii were the most frequently isolated species. Among the cellobiose-fermenting yeasts, 14 known and three novel yeast species were identified. Scheffersomyces queiroziae, Sc. amazonensis, Yamadazyma sp.1, Hanseniaspora opuntiae, C. jaroonii, and Candida tammaniensi s were the main ethanol-producing yeasts. These species also produced an intracellular β-glucosidase responsible for cellobiose hydrolysis. In fermentation assays using a culture medium containing 50 g L −1 cellobiose, ethanol production was observed in all cases; Sc. queiroziae and Sc. amazonensis showed the highest yield, efficiency, and productivity. Candida jaroonii and Yamadazyma sp.1 strains also showed high efficiency in cellobiose fermentation, while C. tammaniensis and H. opuntiae strains produced low amounts of ethanol. This study shows the potential of rotting wood samples from Brazilian ecosystems as a source of yeasts, including new species as well as those with promising biotechnological properties. Highlights: Eighty-six known and 47 novel yeast species were isolated in this study. Scheffersomyces species includes excellent cellobiose fermenters. Yamadazyma species are promising yeasts for use in cellobiose fermentation. In the yeasts studied, cellobiose is metabolised by an intracellular β-glucosidase.