Illumina midi-barcodes: quality proof and applications. (3rd April 2019)

• Record Type: Journal Article
• Authors: Lanner, Julia; Curto, Manuel; Pachinger, Bärbel; Neumüller, Ulrich; Meimberg, Harald
• Is Part Of: Mitochondrial DNA. Volume 30:Number 3(2019)
• Page Start: 490
• Page End: 499
• Publication Date: 2019-04-03
• Subjects: DNA barcoding -- high-throughput sequencing -- Sanger sequencing -- CO1 gene -- wild bees
• DOI: 10.1080/24701394.2018.1551386
• Languages: English
• ISSNs: 2380-2359

Abstract

Abstract: DNA barcoding constitutes a supplemental genetically based characterization tool for the identification of species. Traditionally, the barcodes are generated with a length of 650 bp using standardized Sanger sequencing, but with the introduction of high-throughput sequencing (HTS) methods new opportunities for sequencing are available. To use HTS for barcode collection and identification, the amplification of shorter fragments is preferred. Reference DNA midi-barcodes of wild bees were produced using the Illumina MiSeq as well as the Sanger method. Although DNA midi-barcodes derived from Illumina were comparatively shorter (418 bp), their sequences were coherent to the morphological assignment of species. The Illumina barcodes proved to be effective and dealt better with some general limitations of DNA barcoding.