Electrokinetic potential-stabilization by bile acid-microencapsulating formulation of pancreatic β-cells cultured in high ratio poly-L-ornithine-gel hydrogel colloidal dispersion: applications in cell-biomaterials, tissue engineering and biotechnological applications. (18th August 2018)

• Record Type: Journal Article
• Authors: Mooranian, Armin; Negrulj, Rebecca; Takechi, Ryu; Jamieson, Emma; Morahan, Grant; Al-Salami, Hani
• Is Part Of: Artificial cells, nanomedicine, and biotechnology. Volume 46:Number 6(2018)
• Page Start: 1156
• Page End: 1162
• Publication Date: 2018-08-18
• Subjects: Electrokinetic potential -- tissue engineering -- biomaterials -- polymers; Artificial cells -- Periodicals; Nanotechnology -- Periodicals; Blood substitutes -- Periodicals; Tissue engineering -- Periodicals; Molecules -- Periodicals; Biotechnology -- Periodicals; 615.39
• DOI: 10.1080/21691401.2017.1362416
• Languages: English
• ISSNs: 2169-1401

Abstract

Abstract: Introduction: Current trials for β-cell transplantation have been hindered by poor cell viability and function post-transplantation. Recently, electric charges of the microencapsulating formulation carrying β-cells have shown significant effects on cell survival and function. Thus, this study aimed at investigating the effects of electric charge, of novel colloidal formulation containing β-cells, on cell viability, biological activity and insulin release. Methods : A new formulation, containing high ratios of poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone (1:1:0.1 ratio), was used to form microcapsules utilizing 800 V and 2000 Hz encapsulating conditions. The bile acid, ursodeoxycholic acid, was added into the microcapsules to measure its effects on electric charges. Results: The electric charge of the microencapsulating formulation was enhanced by bile acid addition, and resulted in better cell viability and function. Conclusion: Ursodeoxycholic acid microencapsulated with poly-L-ornithine, suspending electrical-stimulation hydrogel and polystyrene sulphone at 1:1:0.1 ratio, using 800 V and 2000 Hz microencapsulating conditions, produced enhanced electrokinetic parameters of microcapsules with optimized cell functions. This suggests that electric charge of formulations containing pancreatic β-cell may have significant effects on cell mass and functions, post-transplantation.