The Metabolic Profiling of Isorhamnetin-3-O-Neohesperidoside Produced by Human Intestinal Flora Employing UPLC-Q-TOF/MS. (23rd November 2016)

• Record Type: Journal Article
• Authors: Du, Le-yue; Zhao, Min; Tao, Jin-hua; Qian, Da-wei; Jiang, Shu; Shang, Er-xin; Guo, Jian-ming; Liu, Pei; Su, Shu-lan; Duan, Jin-ao
• Is Part Of: Journal of chromatographic science. Volume 55:Number 3(2017:Mar.)
• Page Start: 243
• Page End: 250
• Publication Date: 2016-11-23
• Subjects: Chromatographic analysis -- Periodicals; 543.8
• DOI: 10.1093/chromsci/bmw176
• Languages: English
• ISSNs: 0021-9665

Abstract

Abstract: Isorhamnetin-3- O -neohesperidoside is the major active substance of Puhuang, a traditional herb medicine widely used in clinical practice to tackle many chronic diseases. However, little is known about the interactions between this ingredient and intestinal flora. In this study, ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry together with automated data analysis software (Metabolynx™) was used for analysis of the metabolic profile of isorhamnetin-3- O -neohesperidoside by the isolated human intestinal bacteria. The parent and three metabolites isorhamnetin-3- O -glucoside, isorhamnetin and quercetin were detected and identified based on the characteristics of their deprotonated molecules. These metabolites indicated that isorhamnetin-3- O -neohesperidoside was firstly deglycosylated to isorhamnetin-3- O -glucoside and subsequently to the aglycone isorhamnetin, and the latter was demethylated to quercetin. The majority of bacteria such as Escherichia sp. 23 were capable of converting isorhamnetin-3- O -neohesperidoside to considerable amounts of aglycone isorhamnetin and further to minor amounts of quercetin, while minor amounts of isorhamnetin-3- O -glucoside were detected in minority of bacterial samples such as Enterococcus sp. 3 0. The metabolic pathway and metabolites of isorhamnetin-3- O -neohesperidoside by the different human intestinal bacteria were firstly investigated. Furthermore, the metabolites of isorhamnetin-3- O -neohesperidoside might influence the effects of traditional herb medicines. Thus, our study is helpful to further unravel how isorhamnetin-3- O -neohesperidoside and Puhuang work in vivo .