Identification of Tumor Initiating Cells with a Small‐Molecule Fluorescent Probe by Using Vimentin as a Biomarker. Issue 11 (14th February 2018)

• Record Type: Journal Article
• Authors: Lee, Yong‐An; Kim, Jong‐Jin; Lee, Jungyeol; Lee, Jia Hui Jane; Sahu, Srikanta; Kwon, Haw‐Young; Park, Sung‐Jin; Jang, Se‐Young; Lee, Jun‐Seok; Wang, Zhenxun; Tam, Wai Leong; Lim, Bing; Kang, Nam‐Young; Chang, Young‐Tae
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 11(2018)
• Page Start: 2851
• Page End: 2854
• Publication Date: 2018-02-14
• Subjects: antitumor agents -- biomarkers -- cancer -- fluorescent probes -- high-throughput screening; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201712920
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Tumor initiating cells (TICs) have been implicated in clinical relapse and metastasis of a variety of epithelial cancers, including lung cancer. While efforts toward the development of specific probes for TIC detection and targeting are ongoing, a universal TIC probe has yet to be developed. We report the first TIC‐specific fluorescent chemical probe, TiY, with identification of the molecular target as vimentin, a marker for epithelial‐to‐mesenchymal transition (EMT). TiY selectively stains TICs over differentiated tumor cells or normal cells, and facilitates the visualization and enrichment of functionally active TICs from patient tumors. At high concentration, TiY also shows anti‐TIC activity with low toxicity to non‐TICs. With the unexplored target vimentin, TiY shows potential as a first universal probe for TIC detection in different cancers. Abstract : What makes tumors TIC? The first tumor initiating cell (TIC)‐selective probe, TiY, was developed, with vimentin as the molecular target. TiY facilitates the visualization and enrichment of functionally active TICs from patient‐derived tumors and all of the tested cancer cell lines. Furthermore, TiY showed anti‐TIC activity at high concentrations.