Bioinspired Engineering of a Multivalent Aptamer‐Functionalized Nanointerface to Enhance the Capture and Release of Circulating Tumor Cells. (11th January 2019)

• Record Type: Journal Article
• Authors: Song, Yanling; Shi, Yuanzhi; Huang, Mengjiao; Wang, Wei; Wang, Yang; Cheng, Jie; Lei, Zhichao; Zhu, Zhi; Yang, Chaoyong
• Is Part Of: Angewandte Chemie. Volume 131:Number 8(2019)
• Page Start: 2258
• Page End: 2262
• Publication Date: 2019-01-11
• Subjects: Aptamere -- Nanopartikel -- Mikrofluidik -- Zirkulierende Tumorzellen; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201809337
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Circulating tumor cell (CTC)‐enrichment by using aptamers has a number of advantages, but the issue of compromised binding affinities and stabilities in real samples hinders its wide applications. Inspired by the high efficiency of the prey mechanism of the octopus, we engineered a deterministic lateral displacement (DLD)‐patterned microfluidic chip modified with multivalent aptamer‐functionalized nanospheres (AP‐Octopus‐Chip) to enhance capture efficiency. The multivalent aptamer–antigen binding efficiency improves 100‐fold and the capture efficiency is enhanced more than 300 % compared with a monovalent aptamer‐modified chip. Moreover, the captured cancer cells can be released through a thiol exchange reaction with up to 80 % efficiency and 96 % viability, which is fully compatible with downstream mutation detection and CTC culture. Using the chip, we were able to find CTCs in all cancer samples analyzed. Abstract : Ein aptamermodifizierter Oktopus‐Chip (AP‐Octopus‐Chip) zur Anreicherung von zirkulierenden Tumorzellen (CTCs) wurde entwickelt. Das Design des Chips und die hohe Bindungsaffinität an die Zielzellen verbessern die Effizienz der CTC‐Erfassung und ‐Anreicherung signifikant. Die angereicherten Krebszellen können durch eine Thiolaustauschreaktion freigesetzt werden, die mit dem nachgelagerten Mutationsnachweis und der CTC‐Kultur kompatibel ist.