Pharmacokinetics and tissue distribution of oroxin B in rats using a validated LC–MS/MS assay. (22nd November 2018)

• Record Type: Journal Article
• Authors: Niu, Kai; Yan, Huiyu; Guo, Chunjie; Zhang, Sixi; Zhu, Wei; Teng, Shiyong
• Is Part Of: Biomedical chromatography. Volume 33:Number 3(2019)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-11-22
• Subjects: LC–MS/MS -- oroxin B -- pharmacokinetic study -- tissue distribution; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.4417
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: A highly sensitive LC–MS/MS method was developed to measure oroxin B in rat plasma and tissue homogenates. The analyte and IS were isolated from biological matrices by a simple protein precipitation followed by centrifugation. Detection was conducted by electrospray negative‐ionization mass spectrometry in selected‐reaction monitoring mode. The assay was linear in the concentration range 4.52–904 ng/mL with intra‐ and inter‐day precision of <14.41%. It was successfully applied to the pharmacokinetics and tissue distribution studies of oroxin B after an intravenous dose of 1.0 mg/kg in rats. The results would be useful for further development of oroxin B.