Cytotoxicity analysis of indoor air pollution from biomass combustion in human keratinocytes on a multilayered dynamic cell culture platform. (October 2018)

• Record Type: Journal Article
• Authors: Ke, Shaorui; Liu, Qi; Deng, Mengsi; Zhang, Xinlian; Yao, Yuhan; Shan, Ming; Yang, Xudong; Sui, Guodong
• Is Part Of: Chemosphere. Volume 208(2018)
• Page Start: 1008
• Page End: 1017
• Publication Date: 2018-10
• Subjects: Biomass combustion -- Keratinocytes -- Microfluidics -- Apoptosis -- NF-κB -- Cytotoxicity; Pollution -- Periodicals; Pollution -- Physiological effect -- Periodicals; Environmental sciences -- Periodicals; Atmospheric chemistry -- Periodicals; 551.511
• DOI: 10.1016/j.chemosphere.2018.06.058
• Languages: English
• ISSNs: 0045-6535

Abstract

Abstract: Skin tissue is the first barrier against ambient harmful matter and has direct contact with indoor air pollutants. Nevertheless, a comprehensive understanding of cytotoxicity of indoor air pollution on skin cells is insufficiently clear. Herein, for the first time a multilayered dynamic cell culture platform was established to study the cytotoxicity of indoor air pollutant from biomass combustion in human skin keratinocytes. The platform consisted of seven repetitive polydimethylsiloxane modules carrying six pieces of polycarbonate membrane between them as substrate for cell growth to realize the simultaneous dynamic culture of 12 layers of keratinocytes. After exposure to biomass combustion soluble constituents (BCSCs), cell viability under microfluidic platform conditions declined more significantly, and apoptosis rates increased more obviously compared with well plate conditions. Transmission electron microscope showed that keratinocyte microstructures displayed obvious signs of cellular damage. Our study confirmed that the nuclear factor of kappa B (NF-κB) signaling pathway was activated, which significantly increased the Bax/Bcl-2 ratio and tumor necrosis factor-alpha and interleukin 6 expression, indicating that NF-κB signaling pathway was the major factor in BCSCs-induced cytotoxicity. These findings offer an insight into the mechanism of BCSCs-induced cytotoxicity in keratinocytes and provide a theoretical basis for future studies on skin cells. Graphical abstract: Image 1 Highlights: We fabricated a multilayered dynamic cell culture platform for the first time. The platform was more suitable for investigating the cytotoxicity of BCSCs. We studied the cytotoxicity of BCSCs in keratinocytes in the microfluidic platform. For the first time, we found that BCSCs could activate the NF-κB signaling pathway.