Cryo-EM Analysis Reveals Structural Basis of Helicobacter pylori VacA Toxin Oligomerization. Issue 10 (3rd May 2019)

• Record Type: Journal Article
• Authors: Su, Min; Erwin, Amanda L.; Campbell, Anne M.; Pyburn, Tasia M.; Salay, Lauren E.; Hanks, Jessica L.; Lacy, D. Borden; Akey, David L.; Cover, Timothy L.; Ohi, Melanie D.
• Is Part Of: Journal of molecular biology. Volume 431:Issue 10(2019)
• Page Start: 1956
• Page End: 1965
• Publication Date: 2019-05-03
• Subjects: single-particle cryo-EM -- pore-forming toxins -- gastric cancer; VacA vacuolating cytotoxin A -- cryo-EM cryo-electron microscopy -- FSC Fourier shell correlation; Molecular biology -- Periodicals; Biology -- Periodicals; Biochemistry -- Periodicals; Bacteriology -- Periodicals; Molecular Biology -- Periodicals; Biochemistry -- Periodicals; Biologie moléculaire -- Périodiques; Biologie -- Périodiques; Biochimie -- Périodiques; Moleculaire biologie; Biochemistry; Biology; Molecular biology; Periodicals; 572.805
• DOI: 10.1016/j.jmb.2019.03.029
• Languages: English
• ISSNs: 0022-2836

Abstract

Abstract: Helicobacter pylori colonizes the human stomach and contributes to the development of gastric cancer and peptic ulcer disease. H. pylori secretes a pore-forming toxin called vacuolating cytotoxin A (VacA), which contains two domains (p33 and p55) and assembles into oligomeric structures. Using single-particle cryo-electron microscopy, we have determined low-resolution structures of a VacA dodecamer and heptamer, as well as a 3.8-Å structure of the VacA hexamer. These analyses show that VacA p88 consists predominantly of a right-handed beta-helix that extends from the p55 domain into the p33 domain. We map the regions of p33 and p55 involved in hexamer assembly, model how interactions between protomers support heptamer formation, and identify surfaces of VacA that likely contact membrane. This work provides structural insights into the process of VacA oligomerization and identifies regions of VacA protomers that are predicted to contact the host cell surface during channel formation. Graphical abstract: Unlabelled Image Highlights: Helicobacter pylori VacA activity requires oligomerization of the protein. Cryo-EM was used to determine 3D structures of VacA oligomers. The structures allow us to propose a model for how VacA oligomerizes. The structures allow for prediction of VacA regions that contact host cell membranes. These data provide new insights into the structural basis of VacA activity.