Electrodeposition of a magnetic and redox-active chitosan film for capturing and sensing metabolic active bacteria. (1st September 2018)

• Record Type: Journal Article
• Authors: Li, Ying; Liu, Yi; Kim, Eunkyoung; Song, Yingying; Tsao, Chen-Yu; Teng, Zi; Gao, Tieren; Mei, Lei; Bentley, William E.; Payne, Gregory F.; Wang, Qin
• Is Part Of: Carbohydrate polymers. Volume 195(2018)
• Page Start: 505
• Page End: 514
• Publication Date: 2018-09-01
• Subjects: MNPs magnetic nanoparticles -- MMPs magnetic microparticles -- SEM scanning electron microscopy -- SEM-EDX scanning electron microscopy with energy dispersive X-ray spectroscopy analysis -- VSM vibrating sample magnetometry -- EGFP enhanced green fluorescent protein -- EDC 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide -- Ab antibody -- ms the saturation magnetic moment -- mr remanent magnetic moment -- Chi chitosan -- IP in-plane -- OOP out-of-plane -- Fc Fc/Fcdimethanol -- PYO PYO -- Cat catechol -- E. coli Escherichia coli -- PB phosphate buffer -- CV cyclic voltammograms/cyclic voltammetry -- MF magnetic field -- AR amplification ratio -- RR rectification ratio; Electrodeposition -- Chitosan film -- Magnetic particles -- Redox capacitor -- Multifunction; Polysaccharides -- Periodicals; Polysaccharides -- Periodicals; Polysaccharides -- Périodiques; Electronic journals; 547.78
• DOI: 10.1016/j.carbpol.2018.04.096
• Languages: English
• ISSNs: 0144-8617

Abstract

Graphical abstract: Highlights: An electrochemical method to fabricate a multifunctional hydrogel film was proposed. We enlist a sequence of electrical and magnetic signals for electro-synthesis. The function of the magnetic layer is collecting and concentrating MMP-bacteria. The redox capacitor layer could bridge bacteria-electrode electric communications. Abstract: Rapid and portable detection of viable pathogen is highly desired to minimize the risk of foodborne pathogen outbreaks. Here we report a proof-of-concept fabrication methodology of a multifunctional film that allows established methods from bacterial recognition (antibodies) and nanotechnology (magnetic nanoparticles) to be coupled with electrochemical signal processing methods for detection of viable bacteria. Specifically, we enlist a sequence of externally applied electrical and magnetic signals to: i) guide the self-assembly of stimuli-responsive biopolymer; ii) incorporate magnetic nanoparticles to form a magnetic layer; iii) electro-synthesize a signal processing layer (redox-capacitor). The function of the magnetic layer is collecting and concentrating MMP-bacteria through magnetic attractions between MMP-bacteria and the magnetic layer. The function of the signal processing layer is amplifying electrochemical detection of the collected bacteria by engaging the redox-active mediators with the redox capacitor. Importantly, the fabrication demonstrated here is simple, controllable, and reagentless.