Combinatorial library of chalcogen-containing lipidoids for intracellular delivery of genome-editing proteins. (September 2018)

• Record Type: Journal Article
• Authors: Li, Yamin; Yang, Tao; Yu, Yingjie; Shi, Nicola; Yang, Liu; Glass, Zachary; Bolinger, Justin; Finkel, Isaac James; Li, Wenhan; Xu, Qiaobing
• Is Part Of: Biomaterials. Volume 178(2018)
• Page Start: 652
• Page End: 662
• Publication Date: 2018-09
• Subjects: Lipidoids -- Protein delivery -- CRISPR/Cas9 -- Genome editing; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2018.03.011
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Protein based therapeutics with high specificities and low off-target effects are used for transient and accurate manipulation of cell functions. However, developing safe and efficient carriers for intracellular delivery of active therapeutic proteins is a long-standing challenge. Here we report a combinatorial library of chalcogen (O, S, Se) containing lipidoid nanoparticles (LNPs) as efficient nanocarriers for intracellular delivery of negatively supercharged Cre recombinase ((-30)GFP-Cre) and anionic Cas9:single-guide RNA (Cas9:sgRNA) ribonucleoprotein (RNP) for genome editing. The structure-activity relationship between the lipidoids and intracellular protein delivery efficiencies was explored and it was demonstrated that the newly developed LNPs are effective for gene recombination in vivo . Graphical abstract: Image 1