A mussel polyphenol oxidase-like protein shows thiol-mediated antioxidant activity. (April 2019)

• Record Type: Journal Article
• Authors: Wang, Jia; Suhre, Michael H.; Scheibel, Thomas
• Is Part Of: European polymer journal. Volume 113(2019)
• Page Start: 305
• Page End: 312
• Publication Date: 2019-04
• Subjects: Mussel foot -- Polyphenol oxidase-like protein -- Recombinant production -- Antioxidant -- Free thiols; Polymers -- Periodicals; Polymerization -- Periodicals; Polymères -- Périodiques; Polymérisation -- Périodiques; Polymerization; Polymers; Periodicals; Electronic journals; 547.705
• DOI: 10.1016/j.eurpolymj.2019.01.069
• Languages: English
• ISSNs: 0014-3057

Abstract

Graphical abstract: Highlights: The full-length sequence of polyphenol oxidase- like protein (PPOL) from Mytilus galloprovincialis was identified. Recombinant PPOL (rPPOL) was successfully produced in Escherichia coli . rPPOL exhibits thiol-dependent antioxidant activity suppressing DOPA oxidation. Abstract: Marine mussels adhere underwater to a variety of substrates using adhesive proteins with post-translationally modified amino acids, such as 3, 4-dihydroxyphenylalanine (DOPA) residues, as a key chemical signature. DOPA can auto-oxidize easily in seawater reducing the adhesion strength, but contributing to subsequent cohesion (cross-linking) of the underlying proteins. To maintain both reduced and oxidized forms of DOPA with corresponding adhesion and cohesion properties, strict redox regulation is necessary for mussel underwater adhesion. In this study, a full-length polyphenol oxidase-like protein (PPOL) from Mytilus galloprovincialis was identified after screening of a mussel foot cDNA library using different degenerated PCR primers. The recombinant PPOL (rPPOL) was successfully produced in Escherichia coli . The rPPOL exhibits thiol-dependent antioxidant activity suppressing DOPA oxidation. This finding provides insights into how DOPA chemistry could be regulated and presumably inspires future applications of DOPA-mediated adhesion materials.