A Chemogenetic Approach for the Optical Monitoring of Voltage in Neurons. Issue 8 (25th January 2019)

• Record Type: Journal Article
• Authors: Sundukova, Mayya; Prifti, Efthymia; Bucci, Annalisa; Kirillova, Kseniia; Serrao, Joana; Reymond, Luc; Umebayashi, Miwa; Hovius, Ruud; Riezman, Howard; Johnsson, Kai; Heppenstall, Paul A.
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 8(2019)
• Page Start: 2341
• Page End: 2344
• Publication Date: 2019-01-25
• Subjects: fluorogenic probes -- genetic targeting -- membrane potential probes -- protein tags -- voltage imaging; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201812967
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Optical monitoring of neuronal voltage using fluorescent indicators is a powerful approach for the interrogation of the cellular and molecular logic of the nervous system. Herein, a semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash‐free imaging and faithfully detects supra‐ and sub‐threshold activity in neurons. Abstract : Shine a light : A semisynthetic tethered voltage indicator (STeVI1) based upon nile red is described that displays voltage sensitivity when genetically targeted to neuronal membranes. This environmentally sensitive probe allows for wash‐free imaging and faithfully detects supra‐ and sub‐threshold activity in neurons.