Inhibition of microRNA‐92a increases blood vessels and satellite cells in skeletal muscle but does not improve duchenne muscular dystrophy–related phenotype in mdx mice. Issue 5 (23rd February 2019)

• Record Type: Journal Article
• Authors: Verma, Mayank; Asakura, Yoko; Asakura, Atsushi
• Is Part Of: Muscle & nerve. Volume 59:Issue 5(2019)
• Page Start: 594
• Page End: 602
• Publication Date: 2019-02-23
• Subjects: Antagomir -- endothelial cell -- mdx mouse -- miR‐92a -- muscular dystrophy -- satellite cell; Neuromuscular diseases -- Periodicals; Muscles -- Periodicals; Nerves -- Periodicals; 616.74
• DOI: 10.1002/mus.26433
• Languages: English
• ISSNs: 0148-639X

Abstract

ABSTRACT: Introduction : The vasculature and blood flow in muscle are perturbed in Duchenne muscular dystrophy (DMD) and its mdx mouse model. MicroRNA‐92a (miR‐92a) is enriched in endothelial cells, especially during ischemic injury. Methods : Because antagonizing miR‐92a was shown to result in increased proliferation and migration of endothelial cells and recovery from ischemia, we assessed the effects of Antagomir‐92a in vitro in muscle stem cell culture and in vivo in mdx mice. Results : miR‐92a was found to be highly expressed in muscle endothelial cells and satellite cells. Treatment with Antagomir‐92a increased capillary density and tissue perfusion, which was accompanied by an increase in satellite cells. However, Antagomir‐92a–treated mdx mice showed no histological improvement and had worse muscle function. Antagomir‐92a suppressed myogenic differentiation in satellite cell culture. Discussion : AntagomiR‐92a improves the vasculature but not the muscle in mdx mice, possibly due to its side effects on satellite cell differentiation. Muscle Nerve 59:594–594, 2019