Design and synthesis of dual-excitation fluorescent probe, Tb3+-dtpa-bis(fluorescein), and application in detection of hydrazine in environmental water samples and live cells. (March 2019)

• Record Type: Journal Article
• Authors: Li, Xinyi; Yu, Zhiyue; Li, Na; Jia, Haishuang; Wei, Hao; Wang, Jun; Song, Youtao
• Is Part Of: Dyes and pigments. Volume 162(2019)
• Page Start: 281
• Page End: 294
• Publication Date: 2019-03
• Subjects: Fluorescent probe -- Detection of hydrazine -- Dual-excitation -- Diethylenetriaminepentaacetic acid (dtpa) -- Tb3+-dtpa-bis(fluorescein) complex; Dyes and dyeing -- Periodicals; Pigments -- Periodicals; 667.2
• DOI: 10.1016/j.dyepig.2018.10.044
• Languages: English
• ISSNs: 0143-7208

Abstract

Abstract: A novel dual-excitation fluorescent probe, Tb 3+ -dtpa-bis(fluorescein), for detecting hydrazine is designed based on the special emissions of Tb 3+ -dtpa-BHz and fluorescein at 515 nm and 520 nm, respectively, under 247 nm and 480 nm excitation wavelength lights. The affecting factors including hydrazine concentration, solution pH value and interfering substances on the detection of hydrazine are examined. The high sensitivity and high specificity of Tb 3+ -dtpa-bis(fluorescein) in hydrazine detection could be confirmed. Two good linear responses of fluorescence emission intensities of Tb 3+ -dtpa-bis(fluorescein) at 515 nm and 520 nm as functions of hydrazine concentration in range of 3.00 × 10 −5 mol L −1 - 4.50 × 10 −5 mol L −1 are obtained, respectively. The LOD of 13 ppb (4.08 × 10 −7 mol L −1 ) for 247 nm wavelength light excitation and 8 ppb (2.50 × 10 −7 mol L −1 ) for 480 nm wavelength light excitation are achieved, which are close to and lower than TLV (10 ppb) of the EPA, respectively. The theoretical calculations on HOMO-LUMO energy levels and the interfacial plots of the molecular orbitals for probe and its final product based on density functional theory (DFT) are performed. In addition, the detections of hydrazine in real water samples and living cells are also studied. The experimental results indicated that the developed fluorescent probe could be applied for the determination of hydrazine in environmental water samples and living cells. Graphical abstract: A novel dual-excitation fluorescent probe, Tb 3+ -dtpa-bis(fluorescein), for detecting hydrazine was designed and synthesized based on the reaction of Tb 3+ ion and dtpa-bis(fluorescein) ligand. Tb 3+ -dtpa-bis(fluorescein) can detect hydrazine under excitation of 247 nm and 500 nm wavelength lights. Two good linear responses of fluorescence emission intensities of Tb 3+ -dtpa-bis(fluorescein) at 515 nm and 520 nm as functions of hydrazine concentration in range of 3.00 × 10 −5 mol L −1 - 4.50 × 10 −5 mol L −1 are obtained, respectively. Limits of detection (LOD) of 13 ppb (4.08 × 10 −7 mol L −1 ) for excitation of 247 nm wavelength and 8 ppb (2.50 × 10 −7 mol L −1 ) for excitation wavelength 480 nm were obtained, which are close to and lower than TLV (10 ppb) of the EPA, respectively. In addition, we had also studied the detection of hydrazine in water samples and living cells, it was found that the developed fluorescent probe could be applied for the determination of hydrazine in environmental water samples and living cells. Highlights: Tb 3+ -dtpa-bis(fluorescein) is developed as fluorescent probe to detect hydrazine. Tb 3+ -dtpa-bis(fluorescein) is dual-excitation (247 nm and 480 nm) fluorescent probe. Hydrazinolysis of ester bond enhances Tb 3+ -dtpa-bis(fluorescein) fluorescence signal. Limit of detection (LOD) of hydrazine by Tb 3+ -dtpa-bis(fluorescein) can reach 8 ppb. Tb 3+ -dtpa-bis(fluorescein) can be used to detect hydrazine in real water samples.