High-Frequency Raman Analysis in Biological Tissues Using Dual-Wavelength Excitation Raman Spectroscopy. Issue 2 (February 2020)

• Record Type: Journal Article
• Authors: He, Wencai; Li, Bolan; Yang, Shan
• Is Part Of: Applied spectroscopy. Volume 74:Issue 2(2020)
• Page Start: 241
• Page End: 244
• Publication Date: 2020-02
• Subjects: Raman spectroscopy -- high-frequency Raman -- dual-wavelength excitation -- InGaAs -- hydration probing; Spectrum analysis -- Periodicals; 543.505
• DOI: 10.1177/0003702819881762
• Languages: English
• ISSNs: 0003-7028

Abstract

A dual-wavelength excitation Raman probe with laser inputs at 866 nm or 1064 nm is customized and integrated into a compact Raman spectrometer that is based on an InGaAs detector. Under 1064 nm illumination, the spectrometer detects fingerprint Raman signals below 2000 cm –1 . While under 866 nm illumination, the spectral range is extended to cover high-frequency region (2400–4000 cm –1 ) that includes major C–H and O–H Raman vibrations. We demonstrate that the dual excitation InGaAs Raman is beneficial in detecting high-frequency Raman signals, especially water contents in high-fluorescent biological samples such as human dental tissues, grape skin, and plum skin due to the suppressed fluorescence interference.