Purification, characterization and function analysis of an extracellular β-glucosidase from elongating stipe cell walls in Coprinopsis cinerea. Issue 9 (29th March 2016)

• Record Type: Journal Article
• Authors: Zhang, Wenming; Kang, Liqin; Yang, Mingmei; Zhou, Yajun; Wang, Jun; Liu, Zhonghua; Yuan, Sheng
• Editors: Poeggeler, Stefanie
• Is Part Of: FEMS microbiology letters. Volume 363:Issue 9(2016:May)
• Publication Date: 2016-03-29
• Subjects: extracellular β-glucosidase -- hydrolyase activity -- glycosyltransferase activity -- stipe elongation -- Coprinopsis cinerea; Microbiology -- Periodicals; 579
• DOI: 10.1093/femsle/fnw078
• Languages: English
• ISSNs: 0378-1097

Abstract

Abstract : A β-glycoside hydrolase was isolated from cell walls material in Coprinopsis cinerea elongating stipes. By analysis of SDS-PAGE, MALDI-TOF/TOF MS and substrate specificity, this enzyme was characterized as an extracellular β-glucosidase which is a trimer consisting of three homosubunits. β-Glucosidase did not degrade β-glucans with modified ends, whereas it hydrolyzed various β-glucans with free ends and related oligosaccharides with β-1, 3-, β-1, 4- or β-1, 6-linkages. Although this β-glucosidase possesses glycosyltransferase activity on laminarioligosaccharides, it did not transfer glucose residues from laminaritriose to β-glucan in stipe cell walls to produce larger β-glucan molecules; instead, it caused a decrease in the molecular size of stipe wall β-glucan by removing glucose. Relatively, the molecular size of wall β-glucans in the elongating apical stipe was less than that found in the non-elongating basal stipes, and this β-glucosidase was more highly expressed in the elongating apical stipe than in non-elongating basal regions. Therefore, we propose that β-glucosidase functions by trimming or cutting the β-glucan side chains on the β-1, 3-glucan backbone to prevent them from forming longer branches, keeping the wall plastic to promote diffuse wall growth. Abstract : The β-glucosidase from cell walls of Coprinopsis cinerea caused a decrease in the molecular size of stipe wall β-glucan, and may function by trimming or cutting the β-glucan side chains. Abstract :