The spatiotemporal relationships between chondroitin sulfate proteoglycans and terminations of calcitonin gene related peptide and parvalbumin immunoreactive afferents in the spinal cord of mouse embryos. (10th August 2017)

• Record Type: Journal Article
• Authors: Wang, Liqing; Yu, Chao; Wang, Jun; Zhao, Hui; Chan, Sun-On
• Is Part Of: Neuroscience letters. Volume 655(2017)
• Page Start: 61
• Page End: 67
• Publication Date: 2017-08-10
• Subjects: CSPGs chondroitin sulfate proteoglycans -- CGRP calcitonin gene related peptide -- PV parvalbumin -- DRG dorsal root ganglion -- CNS central nervous system -- IR immunoreactive; Chondroitin sulfate proteoglycans -- Spinal cord -- Development -- Calcitonin gene related peptide -- Parvalbumin; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2017.07.003
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: CSPGs are expressed in a ventral to dorsal gradient in the mouse spinal cord. CGRP immunoreactive axons terminate in regions with weak CS expression. PV immunoreactive axons project to ventral regions of the spinal cord which are rich in CS expression. Abstract: Chondroitin sulfate (CS) proteoglycans (PGs) are a family of complex molecules in the extracellular matrix and cell surface that regulate axon growth and guidance during development of the central nervous system. In this study, the expression of CSPGs was investigated in the mouse spinal cord at late embryonic and neonatal stages using CS-56 antibody. CS immunoreactivity was observed abundantly in ventral regions of spinal cord of embryonic day (E) 15 embryos. At E16 to E18, CS expression spread dorsally, but never reached the superficial layers of the dorsal horn. This pattern was maintained until postnatal day 4, the latest stage examined. Antibodies against calcitonin gene related peptide (CGRP) and parvalbumin (PV) were employed to label primary afferents from nociceptors and proprioceptors, respectively. CGRP-immunoreactive fibers terminated in the superficial regions of the dorsal horn where CSPGs were weakly expressed, whereas PV-immunoreactive fibers were found in CSPG-rich regions in the ventral horn. Therefore, we conclude that CS expression is spatiotemporally regulated in the spinal cord, which correlates to the termination of sensory afferents. This pattern suggests a role of CSPGs on patterning afferents in the spinal cord, probably through a differential response of axons to these growth inhibitory molecules.