A Minimal Sequence for Left‐Handed G‐Quadruplex Formation. Issue 8 (21st January 2019)

• Record Type: Journal Article
• Authors: Bakalar, Blaž; Heddi, Brahim; Schmitt, Emmanuelle; Mechulam, Yves; Phan, Anh Tuân
• Is Part Of: Angewandte Chemie international edition. Volume 58:Issue 8(2019)
• Page Start: 2331
• Page End: 2335
• Publication Date: 2019-01-21
• Subjects: circular dichroism -- DNA -- DNA structures -- G-quadruplexes -- X-ray diffraction; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201812628
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: Recently, we observed the first example of a left‐handed G‐quadruplex structure formed by natural DNA, named Z‐G4 . We analysed the Z‐G4 structure and inspected its primary 28‐nt sequence in order to identify motifs that convey the unique left‐handed twist. Using circular dichroism spectroscopy, NMR spectroscopy, and X‐ray crystallography, we revealed a minimal sequence motif of 12 nt (GTGGTGGTGGTG) for formation of the left‐handed DNA G‐quadruplex, which is found to be highly abundant in the human genome. A systematic analysis of thymine loop mutations revealed a moderate sequence tolerance, which would further broaden the space of sequences prone to left‐handed G‐quadruplex formation. Abstract : It's just a jump to the left : Based on analysis and modification of the two sequence blocks of the natural left‐handed DNA G‐quadruplex Z‐G4, the 12‐nt sequence GTGGTGGTGGTG was identified as a minimal sequence motif for left‐handed G‐quadruplex formation. This motif is abundant in the human genome.