CGMP production and analysis of BG505 SOSIP.664, an extensively glycosylated, trimeric HIV‐1 envelope glycoprotein vaccine candidate. Issue 4 (11th December 2017)

• Record Type: Journal Article
• Authors: Dey, Antu K.; Cupo, Albert; Ozorowski, Gabriel; Sharma, Vaneet K.; Behrens, Anna‐Janina; Go, Eden P.; Ketas, Thomas J.; Yasmeen, Anila; Klasse, Per J.; Sayeed, Eddy; Desaire, Heather; Crispin, Max; Wilson, Ian A.; Sanders, Rogier W.; Hassell, Thomas; Ward, Andrew B.; Moore, John P.
• Is Part Of: Biotechnology and bioengineering. Volume 115:Issue 4(2018)
• Page Start: 885
• Page End: 899
• Publication Date: 2017-12-11
• Subjects: affinity purification -- cGMP -- HIV‐1 vaccine -- native‐like Env trimers -- recombinant vaccine development -- SOSIP; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.26498
• Languages: English
• ISSNs: 0006-3592

Abstract

Abstract: We describe the properties of BG505 SOSIP.664 HIV‐1 envelope glycoprotein trimers produced under current Good Manufacturing Practice (cGMP) conditions. These proteins are the first of a new generation of native‐like trimers that are the basis for many structure‐guided immunogen development programs aimed at devising how to induce broadly neutralizing antibodies (bNAbs) to HIV‐1 by vaccination. The successful translation of this prototype demonstrates the feasibility of producing similar immunogens on an appropriate scale and of an acceptable quality for Phase I experimental medicine clinical trials. BG505 SOSIP.664 trimers are extensively glycosylated, contain numerous disulfide bonds and require proteolytic cleavage, all properties that pose a substantial challenge to cGMP production. Our strategy involved creating a stable CHO cell line that was adapted to serum‐free culture conditions to produce envelope glycoproteins. The trimers were then purified by chromatographic methods using a 2G12 bNAb affinity column and size‐exclusion chromatography. The chosen procedures allowed any adventitious viruses to be cleared from the final product to the required extent of >12 log10 . The final cGMP production run yielded 3.52 g (peptidic mass) of fully purified trimers (Drug Substance) from a 200 L bioreactor, a notable yield for such a complex glycoprotein. The purified trimers were fully native‐like as judged by negative‐stain electron microscopy, and were stable over a multi‐month period at room temperature or below and for at least 1 week at 50° C. Their antigenicity, disulfide bond patterns, and glycan composition were consistent with trimers produced on a research laboratory scale. The methods reported here should pave the way for the cGMP production of other native‐like Env glycoprotein trimers of various designs and genotypes. Abstract : The paper describes the production and properties of the BG505 SOSIP.664 HIV‐1 envelope glycoprotein trimer under cGMP conditions. This complex, highly glycosylated and conformationally sensitive protein complex is the first of a new generation of immunogens designed to mimic the structure of the envelope spikes present on the surface of HIV‐1 virus particles.