Mycobacterium tuberculosis H37Rv infection regulates alternative splicing in Macrophages. Issue 1 (1st January 2018)

• Record Type: Journal Article
• Authors: Zhang, Wei; Niu, Chen; Fu, Rui-Yang; Peng, Zheng-Yu
• Is Part Of: Bioengineered. Volume 9:Issue 1(2018)
• Page Start: 203
• Page End: 208
• Publication Date: 2018-01-01
• Subjects: alternative splicing -- IL4 -- Mycobacterium tuberculosis -- SR proteins -- TLR4; Biomedical engineering -- Periodicals; Biotechnology -- Periodicals; Microbiology -- Periodicals; 660.6
• DOI: 10.1080/21655979.2017.1387692
• Languages: English
• ISSNs: 2165-5987

Abstract

ABSTRACT: Objective: The objective of this study was to evaluate the expression of genes encoding SR proteinsand alternative splicing of IL4 and TLR4 in Mycobacterium tuberculosis (M. tb) H37Rv-infected macrophages. Materials and methods: THP-1 cells were induced to differentiate into macrophages with 200 nM PMA, and H37Rv strains were used for macrophage infection. After RNA extraction, qRT-PCR was performed to evaluate the expression of many SR proteins as well as the alternative splicing of IL4 and TLR4. Results: IL4 and TLR4 play significant roles in host immunity to tuberculosis. The level of IL-4 splice variants in THP-1 cells increased after M. tb H37Rv infection. Three splice variants of TLR4 were detected in M. tb -infected THP-1 cells, when compared with uninfected controls; the expression level of these splicing variants in M. tb -infected THP-1 cell was down-regulated. Since SR proteins are RNA-binding proteins that regulate RNA splicing, the expression of SR proteins was examined, and SRSF2 and SRSF3 were significantly down-regulated. In addition, splicing factors SRp75 and SF3a were also significantly down-regulated post M. tb infection. Conclusion: Our findings indicate that alternative splicing may be involved in host gene regulation post M. tb infection of macrophage cells.